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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F10%3A00350141%21RIV11-GA0-61388971
rdf:type
n7:Vysledek skos:Concept
dcterms:description
The genetic diversity of streptomycetes in colliery spoil heaps (Sokolov), Czech Republic) was investigated by restriction pattern analysis of 165-internal transcribed spacer rDNA and 165 sequences. We sampled freshly excavated Miocene sediment (17-19-million-year-old) and four sites of primary succession (initial, early, middle, and late stages; aged 1-44 years) on the same sediment. Active bacteria were present even in fresh Miocene sediment, and the relative proportion of actinomycetes among total bacterial and their genetic diversity increased significantly with the age of the sampling site. The replacement of pioneer species by late succession species during succession was observed. Plate assays of Streptomyces strains revealed 27% antibiotic-producing strains. Screening for nonribosomal peptide synthases and type I polyketide synthases systems suggested that 90% and 55% streptomycetes, respectively, are putative producers of biologically active compounds. The genetic diversity of streptomycetes in colliery spoil heaps (Sokolov), Czech Republic) was investigated by restriction pattern analysis of 165-internal transcribed spacer rDNA and 165 sequences. We sampled freshly excavated Miocene sediment (17-19-million-year-old) and four sites of primary succession (initial, early, middle, and late stages; aged 1-44 years) on the same sediment. Active bacteria were present even in fresh Miocene sediment, and the relative proportion of actinomycetes among total bacterial and their genetic diversity increased significantly with the age of the sampling site. The replacement of pioneer species by late succession species during succession was observed. Plate assays of Streptomyces strains revealed 27% antibiotic-producing strains. Screening for nonribosomal peptide synthases and type I polyketide synthases systems suggested that 90% and 55% streptomycetes, respectively, are putative producers of biologically active compounds.
dcterms:title
Biodiversity of streptomycetes isolated from a succession sequence at a post-mining site and their evidence in Miocene lacustrine sediment Biodiversity of streptomycetes isolated from a succession sequence at a post-mining site and their evidence in Miocene lacustrine sediment
skos:prefLabel
Biodiversity of streptomycetes isolated from a succession sequence at a post-mining site and their evidence in Miocene lacustrine sediment Biodiversity of streptomycetes isolated from a succession sequence at a post-mining site and their evidence in Miocene lacustrine sediment
skos:notation
RIV/61388971:_____/10:00350141!RIV11-GA0-61388971
n3:aktivita
n6:V n6:Z n6:P
n3:aktivity
P(2B06154), P(GA526/03/1259), P(IAA600660607), P(LC06066), V, Z(AV0Z50200510), Z(AV0Z60660521)
n3:cisloPeriodika
7
n3:dodaniDat
n13:2011
n3:domaciTvurceVysledku
n15:6704158
n3:druhVysledku
n17:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
248854
n3:idVysledku
RIV/61388971:_____/10:00350141
n3:jazykVysledku
n16:eng
n3:klicovaSlova
actinomycetes; antibiotics; primary succession
n3:klicoveSlovo
n11:antibiotics n11:primary%20succession n11:actinomycetes
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[93A755582A62]
n3:nazevZdroje
Microbiological Research
n3:obor
n4:EH
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
4
n3:projekt
n8:GA526%2F03%2F1259 n8:2B06154 n8:LC06066 n8:IAA600660607
n3:rokUplatneniVysledku
n13:2010
n3:svazekPeriodika
165
n3:tvurceVysledku
Krištůfek, Václav Elhottová, Dana Chroňáková, Alica Tichý, Martin
n3:wos
000282740100008
n3:zamer
n9:AV0Z50200510 n9:AV0Z60660521
s:issn
0944-5013
s:numberOfPages
15