This HTML5 document contains 60 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n12http://linked.opendata.cz/resource/domain/vavai/projekt/
n8http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n16http://linked.opendata.cz/ontology/domain/vavai/
n7http://linked.opendata.cz/resource/domain/vavai/zamer/
n17http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F61388971%3A_____%2F09%3A00337531%21RIV10-MSM-61388971/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n13http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n14http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n15http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n10http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n5http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F09%3A00337531%21RIV10-MSM-61388971
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Paper presents a complete sample handling protocol, which allows processing of disulfide containing proteins at basic pH. We modified the standard SDS gel electrophoresis and protein digestion conditions by the addition of an oxidative agent, cystamine. Thismodification prevented disulfide scrambling, which we otherwise observed in the samples handled according to the general protocol. Lysozyme from hen egg was used as a model protein for the development of the method. Disulfide bonds were characterized in the following proteins - human leukocyte antigen CD69, murine leukocyte receptor NKR-P1A and glycosylated β-N-acetylhexosaminidases from Aspergillus oryzae and Penicillium oxalicum Paper presents a complete sample handling protocol, which allows processing of disulfide containing proteins at basic pH. We modified the standard SDS gel electrophoresis and protein digestion conditions by the addition of an oxidative agent, cystamine. Thismodification prevented disulfide scrambling, which we otherwise observed in the samples handled according to the general protocol. Lysozyme from hen egg was used as a model protein for the development of the method. Disulfide bonds were characterized in the following proteins - human leukocyte antigen CD69, murine leukocyte receptor NKR-P1A and glycosylated β-N-acetylhexosaminidases from Aspergillus oryzae and Penicillium oxalicum
dcterms:title
Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds
skos:prefLabel
Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds
skos:notation
RIV/61388971:_____/09:00337531!RIV10-MSM-61388971
n4:aktivita
n14:P n14:Z
n4:aktivity
P(IAA5020403), P(KJB400200501), P(KJB500200612), P(LC07017), P(LC545), Z(AV0Z50200510), Z(MSM0021620808), Z(MSM0021620835), Z(MSM6198959216)
n4:cisloPeriodika
11
n4:dodaniDat
n5:2010
n4:domaciTvurceVysledku
n8:8710740 n8:8732124 n8:8652406 n8:9259619 n8:1810561 n8:7690088 n8:4576462 n8:4140702
n4:druhVysledku
n15:J
n4:duvernostUdaju
n18:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
327130
n4:idVysledku
RIV/61388971:_____/09:00337531
n4:jazykVysledku
n9:eng
n4:klicovaSlova
disulfide bond; cystamine; gel electrophoresis
n4:klicoveSlovo
n13:gel%20electrophoresis n13:cystamine n13:disulfide%20bond
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[8368F72B5D35]
n4:nazevZdroje
Journal of Mass Spectrometry
n4:obor
n10:CE
n4:pocetDomacichTvurcuVysledku
8
n4:pocetTvurcuVysledku
8
n4:projekt
n12:LC545 n12:KJB400200501 n12:IAA5020403 n12:KJB500200612 n12:LC07017
n4:rokUplatneniVysledku
n5:2009
n4:svazekPeriodika
44
n4:tvurceVysledku
Hofbauerová, Kateřina Kumar, Vinay Havlíček, Vladimír Pompach, Petr Bezouška, Karel Man, Petr Kavan, Daniel Novák, Petr
n4:wos
000272150300004
n4:zamer
n7:MSM6198959216 n7:MSM0021620808 n7:MSM0021620835 n7:AV0Z50200510
s:issn
1076-5174
s:numberOfPages
8