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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F09%3A00330788%21RIV10-AV0-61388971
rdf:type
skos:Concept n14:Vysledek
dcterms:description
This paper reports on expression, ioslation and testing of protease type XVIII from Rhizopus sp. Recombinant protease proved to be much more efficient than the commercially availabe crude extract. Moreover it showed excellent cleavage specifity and efficiency when immobilized to the POROS resin. The use of this protease enhance spatial resolution achievable in hydrogen/deuterium exchange coupled to mass spectrometry This paper reports on expression, ioslation and testing of protease type XVIII from Rhizopus sp. Recombinant protease proved to be much more efficient than the commercially availabe crude extract. Moreover it showed excellent cleavage specifity and efficiency when immobilized to the POROS resin. The use of this protease enhance spatial resolution achievable in hydrogen/deuterium exchange coupled to mass spectrometry
dcterms:title
Recombinant immobilized rhizopuspepsin as a new tool for protein digestion in hydrogen/deuterium exchange mass spectrometry Recombinant immobilized rhizopuspepsin as a new tool for protein digestion in hydrogen/deuterium exchange mass spectrometry
skos:prefLabel
Recombinant immobilized rhizopuspepsin as a new tool for protein digestion in hydrogen/deuterium exchange mass spectrometry Recombinant immobilized rhizopuspepsin as a new tool for protein digestion in hydrogen/deuterium exchange mass spectrometry
skos:notation
RIV/61388971:_____/09:00330788!RIV10-AV0-61388971
n5:aktivita
n11:Z
n5:aktivity
Z(AV0Z50200510)
n5:cisloPeriodika
21
n5:dodaniDat
n6:2010
n5:domaciTvurceVysledku
n12:9259619
n5:druhVysledku
n17:J
n5:duvernostUdaju
n7:S
n5:entitaPredkladatele
n9:predkladatel
n5:idSjednocenehoVysledku
338383
n5:idVysledku
RIV/61388971:_____/09:00330788
n5:jazykVysledku
n13:eng
n5:klicovaSlova
mass spectrometry; recombinant immobilized rhizopuspepsin; protease
n5:klicoveSlovo
n8:protease n8:recombinant%20immobilized%20rhizopuspepsin n8:mass%20spectrometry
n5:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n5:kontrolniKodProRIV
[1B85D4649C87]
n5:nazevZdroje
Rapid Communications in Mass Spectrometry
n5:obor
n15:CE
n5:pocetDomacichTvurcuVysledku
1
n5:pocetTvurcuVysledku
5
n5:rokUplatneniVysledku
n6:2009
n5:svazekPeriodika
23
n5:tvurceVysledku
Brandolin, G. Rey, M. Man, Petr Forest, E. Pelosi, L.
n5:wos
000271431500010
n5:zamer
n16:AV0Z50200510
s:issn
0951-4198
s:numberOfPages
8