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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F08%3A00350955%21RIV11-MSM-61388971
rdf:type
skos:Concept n19:Vysledek
dcterms:description
A new program NovaQ was developed for precise protein quantitation by mass spectrometry and SILAC labeling. The program works under Microsoft Wingows XP as well as MacOS patforms. By using peptide mass fingerprinting with 1.5 ppm mass tolerance we were able to utilize far more data retained in MS scans which would normally be missed by a standard processing method. This new way of data interpretation results in an improvement of the relevance of quantitation experiments and enabled us to search and quantify different types of post-translational modifications. Furthermore, we used this technique to distinguish among different protein isoforms, commonly returned by Mascot search engine A new program NovaQ was developed for precise protein quantitation by mass spectrometry and SILAC labeling. The program works under Microsoft Wingows XP as well as MacOS patforms. By using peptide mass fingerprinting with 1.5 ppm mass tolerance we were able to utilize far more data retained in MS scans which would normally be missed by a standard processing method. This new way of data interpretation results in an improvement of the relevance of quantitation experiments and enabled us to search and quantify different types of post-translational modifications. Furthermore, we used this technique to distinguish among different protein isoforms, commonly returned by Mascot search engine
dcterms:title
Program NovaQ Program NovaQ
skos:prefLabel
Program NovaQ Program NovaQ
skos:notation
RIV/61388971:_____/08:00350955!RIV11-MSM-61388971
n3:aktivita
n9:P n9:Z
n3:aktivity
P(LC07017), Z(AV0Z50200510)
n3:dodaniDat
n13:2011
n3:domaciTvurceVysledku
n5:9259619 n5:2194538 n5:8710740 n5:4576462 n5:8652406 n5:4316851 n5:4140702
n3:druhVysledku
n6:R
n3:duvernostUdaju
n14:S
n3:ekonomickeParametry
Program poskytuje přesnější kvantitativní stanovení bílkovin
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
390213
n3:idVysledku
RIV/61388971:_____/08:00350955
n3:interniIdentifikace
FVZ/MBU/MS-Quantitation01
n3:jazykVysledku
n17:eng
n3:klicovaSlova
NovaQ; protein; mass spectrometry
n3:klicoveSlovo
n4:protein n4:NovaQ n4:mass%20spectrometry
n3:kontrolniKodProRIV
[952346E6A241]
n3:licencniPoplatek
n18:A
n3:lokalizaceVysledku
Mikrobiologický ústav AV ČR v.v, Vídeňská 1083, 142 20 Praha 4-Krč
n3:obor
n15:EE
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
7
n3:projekt
n11:LC07017
n3:rokUplatneniVysledku
n13:2008
n3:technickeParametry
Program pracuje na platformě Windows XP (Microsoft Corporation) a MacOS
n3:tvurceVysledku
Lemr, Karel Pompach, Petr Man, Petr Havlíček, Vladimír Novák, Petr Strohalm, Martin Kavan, Daniel
n3:vlastnik
n7:vlastnikVysledku
n3:vyuzitiJinymSubjektem
n10:A
n3:zamer
n12:AV0Z50200510