This HTML5 document contains 47 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n16http://linked.opendata.cz/resource/domain/vavai/projekt/
n18http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F61388971%3A_____%2F07%3A00096764%21RIV08-AV0-61388971/
n7http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n5http://linked.opendata.cz/ontology/domain/vavai/
n13http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n11http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n17http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n15http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n6http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n10http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F07%3A00096764%21RIV08-AV0-61388971
rdf:type
n5:Vysledek skos:Concept
dcterms:description
Streptomyces caelestis DSM 40 084 produces two osmolytes, viz. 2-O-(D-glucopyranosyl)-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3 procent NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells, its concentration reached 3 g/L during the cultivation in a yeast extract–(NH4)2SO4–glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium Streptomyces caelestis DSM 40 084 produces two osmolytes, viz. 2-O-(D-glucopyranosyl)-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3 procent NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells, its concentration reached 3 g/L during the cultivation in a yeast extract–(NH4)2SO4–glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium Streptomyces caelestis DSM 40 084 produkuje dva osmolyty: 2-O-(D-glukopyranosyl-glycerolovou kyselinu (GG) a trehalosu. Obě sloučeniny byly izolovány a identifikovány nukleární magnetickou resonancí a hmotnostní spektrometrií. Byla zjištěna velmi citlivá regulace buněčných osmolytů v exponenciálně rostoucích kulturách. Intracelulární obsah GG a trehalosy se zvýšil dvakrát po skokové změně osmolarity média vyvolané 0.3 procent NaCl. 1H NMR analýza buněčných extraktů nepotvrdila přítomnost dalších osmolytů. GG je metabolit uvolňovaný z buněk a jeho koncentrace dosahuje 3g/L při kultivaci v médiu obsahující yeast extrakt, (NH4)2SO4 a glycerol. Tato práce poprvé informuje o výskytu iontového osmolytu GG u rodu Streptomyces a o jeho volné exkreci do média
dcterms:title
Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis Glukosylglycerát je osmolit a extracelulární metabolit produkovaný Streptomyces caelestis Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis
skos:prefLabel
Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis Glukosylglycerát je osmolit a extracelulární metabolit produkovaný Streptomyces caelestis
skos:notation
RIV/61388971:_____/07:00096764!RIV08-AV0-61388971
n4:strany
451;456
n4:aktivita
n9:P n9:Z
n4:aktivity
P(IAA600660607), Z(AV0Z50200510)
n4:cisloPeriodika
5
n4:dodaniDat
n10:2008
n4:domaciTvurceVysledku
n7:1569015 n7:3627586 n7:2185148 n7:7248164
n4:druhVysledku
n6:J
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
423486
n4:idVysledku
RIV/61388971:_____/07:00096764
n4:jazykVysledku
n17:eng
n4:klicovaSlova
streptomyces caelestis; mass spectrometry
n4:klicoveSlovo
n11:mass%20spectrometry n11:streptomyces%20caelestis
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[CE726D1D94D9]
n4:nazevZdroje
Folia Microbiologica
n4:obor
n15:EE
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
4
n4:projekt
n16:IAA600660607
n4:rokUplatneniVysledku
n10:2007
n4:svazekPeriodika
52
n4:tvurceVysledku
Petříček, Miroslav Halada, Petr Pospíšil, Stanislav Sedmera, Petr
n4:zamer
n13:AV0Z50200510
s:issn
0015-5632
s:numberOfPages
6