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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F07%3A00090142%21RIV08-AV0-61388971
rdf:type
skos:Concept n11:Vysledek
dcterms:description
The gene encoding an epoxide hydrolase from Aspergillus niger M200 has been clonek and its sequence determined. Saturation mutagenesis at codon 217 resulted in the discovery of nine enzyme variants showing in several cases profound differences in activity and enantioselectivity towards various epoxides when compared to the data of the wild-type enzyme. The site 217 is located at the entrance of the tunnel that provides the substrate with access to the active site Gen kódující epoxidhydrolasu z Aspergillus niger M200 byl klonován. Saturační mutageneze kodonu 217 vedla k objevení devíti variant enzymu (v některých případech) s výraznými rozdíly v aktivitě a enantioselektivitě (po porovnání s původním nezměněným enzymem). Pozice 217 je lokalizována u vchodu do substrátového tunelu, který spojuje aktivní centrum enzymu s vnějškem The gene encoding an epoxide hydrolase from Aspergillus niger M200 has been clonek and its sequence determined. Saturation mutagenesis at codon 217 resulted in the discovery of nine enzyme variants showing in several cases profound differences in activity and enantioselectivity towards various epoxides when compared to the data of the wild-type enzyme. The site 217 is located at the entrance of the tunnel that provides the substrate with access to the active site
dcterms:title
Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering Klonování genu kódujícího epoxidhydrolasu z Aspergillus niger M200, nadprodukce v E. coli a modifikace aktivity a enantioselectivity enzymu proteinovým inženýrstvím
skos:prefLabel
Klonování genu kódujícího epoxidhydrolasu z Aspergillus niger M200, nadprodukce v E. coli a modifikace aktivity a enantioselectivity enzymu proteinovým inženýrstvím Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering
skos:notation
RIV/61388971:_____/07:00090142!RIV08-AV0-61388971
n3:strany
8;15
n3:aktivita
n10:P n10:Z
n3:aktivity
P(GA204/06/0458), Z(AV0Z50200510)
n3:cisloPeriodika
-
n3:dodaniDat
n13:2008
n3:domaciTvurceVysledku
n6:2634007 n6:9710752 n6:7913680 n6:8710678
n3:druhVysledku
n8:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
413924
n3:idVysledku
RIV/61388971:_____/07:00090142
n3:jazykVysledku
n5:eng
n3:klicovaSlova
protein engineering; saturation mutagenesis; enantioselectivity
n3:klicoveSlovo
n9:enantioselectivity n9:protein%20engineering n9:saturation%20mutagenesis
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[992DFE955C7C]
n3:nazevZdroje
Journal of Biotechnology
n3:obor
n17:EE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n18:GA204%2F06%2F0458
n3:rokUplatneniVysledku
n13:2007
n3:svazekPeriodika
132
n3:tvurceVysledku
Kyslík, Pavel Kotík, Michael Marešová, Helena Štěpánek, Václav
n3:zamer
n15:AV0Z50200510
s:issn
0168-1656
s:numberOfPages
8