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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F07%3A00088898%21RIV08-AV0-61388971
rdf:type
skos:Concept n11:Vysledek
dcterms:description
An extracellular alfa-D-galactosidase from Talaromyces flavus CCF 2686 with extremely broad and unusual acceptor specificity is produced exclusively in the presence of the specific inducer - 6-deoxy-D-glucose (quinovose). The procedure for the preparation of this very expensive substance has been modified and optimized. Surprisingly, any of other common alfa-D-galactosidase inducers or substrates, e.g., D-galactose, melibiose and raffinose, did not stimulate its production. The crude alfa-D-galactosidase preparation was purified by anion-exchange chromatography and three isoenzymes with different substrate specificities were identified Extracelulární alfa-D-galaktosidasa z Talaromyces flavus CCF 2686 s extrémě širokou a neobvyklou substrátovou specifitou je produkována výhradně v přítomnosti specifického induktoru - 6-deoxy-D-glukosy (quinovosa). Enzym byl purifikován aniontově-výměnnou chromatografií a byly identifikovány 3 isoenzymy s rozdílnou substrátovou specifitou An extracellular alfa-D-galactosidase from Talaromyces flavus CCF 2686 with extremely broad and unusual acceptor specificity is produced exclusively in the presence of the specific inducer - 6-deoxy-D-glucose (quinovose). The procedure for the preparation of this very expensive substance has been modified and optimized. Surprisingly, any of other common alfa-D-galactosidase inducers or substrates, e.g., D-galactose, melibiose and raffinose, did not stimulate its production. The crude alfa-D-galactosidase preparation was purified by anion-exchange chromatography and three isoenzymes with different substrate specificities were identified
dcterms:title
Indukce a charakterizace neobvyklé Alfa-D-galaktosidasy z Talaromyces flavus Induction and characterization of an unusual Alfa-D-galactosidase from Talaromyces flavus Induction and characterization of an unusual Alfa-D-galactosidase from Talaromyces flavus
skos:prefLabel
Induction and characterization of an unusual Alfa-D-galactosidase from Talaromyces flavus Indukce a charakterizace neobvyklé Alfa-D-galaktosidasy z Talaromyces flavus Induction and characterization of an unusual Alfa-D-galactosidase from Talaromyces flavus
skos:notation
RIV/61388971:_____/07:00088898!RIV08-AV0-61388971
n3:strany
61;71
n3:aktivita
n6:P n6:N n6:Z
n3:aktivity
N, P(GA203/05/0172), P(LC06010), P(OC D25.001), P(OC D25.002), Z(AV0Z50200510)
n3:cisloPeriodika
1
n3:dodaniDat
n13:2008
n3:domaciTvurceVysledku
n9:8073392 n9:5894190 n9:8732124 n9:1724452 n9:7346689
n3:druhVysledku
n18:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
426027
n3:idVysledku
RIV/61388971:_____/07:00088898
n3:jazykVysledku
n12:eng
n3:klicovaSlova
alfa-d-galactosidase; talaromyces flavus; enzyme purification
n3:klicoveSlovo
n7:alfa-d-galactosidase n7:enzyme%20purification n7:talaromyces%20flavus
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[E3321360030F]
n3:nazevZdroje
Journal of Biotechnology
n3:obor
n4:EE
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
8
n3:projekt
n10:LC06010 n10:GA203%2F05%2F0172 n10:OC%20D25.002 n10:OC%20D25.001
n3:rokUplatneniVysledku
n13:2007
n3:svazekPeriodika
128
n3:tvurceVysledku
Monti, D. Křen, Vladimír Čechová, Ivana Bezouška, Karel Riva, S. Macková, M. Pelantová, Helena Simerská, Pavla
n3:zamer
n16:AV0Z50200510
s:issn
0168-1656
s:numberOfPages
11