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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F05%3A00022301%21RIV06-AV0-61388971
rdf:type
skos:Concept n17:Vysledek
dcterms:description
A high-yield preparatory procedure for the synthesis of p-nitrophenyl 2-acetamido-2-deoxy-b-dgalacto-hexodialdo-1,5-pyranoside (2) using the galactose oxidase from Dactylium dendroides in a batch reactor was developed. Enzymatic recognition of this aldehyde and the respective uronic acid 3 obtained by NaClO2 oxidation was studied using a set of 36 fungal b-N-acetylhexosaminidases from Acremonium, Aspergillus, Penicillium and Talaromyces genera. The aldehyde 2 was readily hydrolysed by all tested b-Nacetylhexosaminidases but neither the uronic acid 3 nor its methyl ester 4 were accepted. Molecular modelling with docking into the active centre of the b-Nacetylhexosaminidase from Aspergillus oryzae revealed that the aldehyde 2 is processed as a C-6 geminal diol by the enzyme Byla vyvinuta vysoce účinná metoda pro syntézu 2-acetamido-2-deoxy-b-D-galakto-hexodialdo-1,5-pyranosidu (látka 2) s použitím galaktosaoxidasy z Dactylium dendroides a vsádkového reaktoru. Enzymatické rozpoznání tohoto aldehydu a též odpovídající kyseliny uronové (látka 3) získané oxidací NaClO2 bylo studováno na souboru 36 plísňových beta-N-acetylhexosaminidas z druhů Acremonium, Penicillium a Talaromyces. Aldehyd 2 byl snadno hydrolyzován všemi testovanými enzymy, ale ani kyselina uronová 3 ani její methyl ester nebyly akceptovány. Molekulární modelování do struktury aktivního centra hexosaminidasy z Aspergillus oryzae odhalilo, že aldehyd 2 je pravděpodobně enzymem zpracováván ve formě C-6 geminálního diolu A high-yield preparatory procedure for the synthesis of p-nitrophenyl 2-acetamido-2-deoxy-b-dgalacto-hexodialdo-1,5-pyranoside (2) using the galactose oxidase from Dactylium dendroides in a batch reactor was developed. Enzymatic recognition of this aldehyde and the respective uronic acid 3 obtained by NaClO2 oxidation was studied using a set of 36 fungal b-N-acetylhexosaminidases from Acremonium, Aspergillus, Penicillium and Talaromyces genera. The aldehyde 2 was readily hydrolysed by all tested b-Nacetylhexosaminidases but neither the uronic acid 3 nor its methyl ester 4 were accepted. Molecular modelling with docking into the active centre of the b-Nacetylhexosaminidase from Aspergillus oryzae revealed that the aldehyde 2 is processed as a C-6 geminal diol by the enzyme
dcterms:title
Combined Application of Galactose Oxidase and Beta-N-Acetylhexosaminidase in the Synthesis of Complex Immunoactive N-Acetyl-D-galactosaminides Combined Application of Galactose Oxidase and Beta-N-Acetylhexosaminidase in the Synthesis of Complex Immunoactive N-Acetyl-D-galactosaminides Použití galaktosaoxidasy v kombinaci s beta-N-acetylhexosaminidasou pro syntézu komplexních imunoaktivních N-acetyl-D-galaktosaminidů
skos:prefLabel
Combined Application of Galactose Oxidase and Beta-N-Acetylhexosaminidase in the Synthesis of Complex Immunoactive N-Acetyl-D-galactosaminides Combined Application of Galactose Oxidase and Beta-N-Acetylhexosaminidase in the Synthesis of Complex Immunoactive N-Acetyl-D-galactosaminides Použití galaktosaoxidasy v kombinaci s beta-N-acetylhexosaminidasou pro syntézu komplexních imunoaktivních N-acetyl-D-galaktosaminidů
skos:notation
RIV/61388971:_____/05:00022301!RIV06-AV0-61388971
n3:strany
997;1006
n3:aktivita
n9:P n9:Z
n3:aktivity
P(GA203/04/1045), Z(AV0Z50200510), Z(MSM6007665808)
n3:cisloPeriodika
-
n3:dodaniDat
n4:2006
n3:domaciTvurceVysledku
n5:8732124 n5:9966366 n5:3895831 n5:1927469 n5:5894190 n5:7203993 n5:1431218
n3:druhVysledku
n13:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
515698
n3:idVysledku
RIV/61388971:_____/05:00022301
n3:jazykVysledku
n18:eng
n3:klicovaSlova
galactose oxidase; glycosylation
n3:klicoveSlovo
n16:galactose%20oxidase n16:glycosylation
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[116A64303E0E]
n3:nazevZdroje
Advanced Synthesis & Catalysis
n3:obor
n8:EE
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
10
n3:projekt
n10:GA203%2F04%2F1045
n3:rokUplatneniVysledku
n4:2005
n3:svazekPeriodika
347
n3:tvurceVysledku
Křenek, Karel Ettrich, R. Namdjou, D.-J. Rauvolfová, Jana Elling, L. Křen, Vladimír Bezouška, Karel Kuzma, Marek Přikrylová, Věra Fialová, Pavla
n3:zamer
n7:AV0Z50200510 n7:MSM6007665808
s:issn
1615-4150
s:numberOfPages
10