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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F04%3A00107943%21RIV%2F2005%2FMSM%2FA53005%2FN
rdf:type
n7:Vysledek skos:Concept
dcterms:description
The trisaccharides GlcNAc-beta(1-4)-GlcNAc-beta(1-4)ManNAc (1) and GalNAc-beta(1-4)-GlcNAc-beta(1-4)ManNAc (2) were synthetised using beta-N-acetylhexosaminidase from Aspergillus oryzae. NMR and molecular modelling proved that both compounds are recognised by protein hevein, similarly to chitotriose (3). The association constants decrease in order 3, 1, 2. The trisaccharides GlcNAc-beta(1-4)-GlcNAc-beta(1-4)ManNAc (1) and GalNAc-beta(1-4)-GlcNAc-beta(1-4)ManNAc (2) were synthetised using beta-N-acetylhexosaminidase from Aspergillus oryzae. NMR and molecular modelling proved that both compounds are recognised by protein hevein, similarly to chitotriose (3). The association constants decrease in order 3, 1, 2. Pomocí beta-N-acatylhexosaminidázy z Aspergillus oryzae byly syntetizovány trisacharidy GlcNAc-beta(1-4)-GlcNAc-beta(1-4)ManNAc (1) a GalNAc-beta(1-4)-GlcNAc-beta(1-4)ManNAc (2). Jejich vazba na protein hevein byla spolu s chitobiosou (3) prokázána NMR a molekulárním modelováním. Asociační konstanty klesají v pořadí 3, 1, 2.
dcterms:title
Enzymatická syntéza glykozaminotrióz a jejich molekulární identifikace pomocí herein domén Enzymatic synthesis of complex glycosaminotrioses and study of their molecular recognition by hevein domains Enzymatic synthesis of complex glycosaminotrioses and study of their molecular recognition by hevein domains
skos:prefLabel
Enzymatic synthesis of complex glycosaminotrioses and study of their molecular recognition by hevein domains Enzymatic synthesis of complex glycosaminotrioses and study of their molecular recognition by hevein domains Enzymatická syntéza glykozaminotrióz a jejich molekulární identifikace pomocí herein domén
skos:notation
RIV/61388971:_____/04:00107943!RIV/2005/MSM/A53005/N
n3:strany
1987;1994
n3:aktivita
n14:P
n3:aktivity
P(GA203/04/1045), P(OC D25.002)
n3:cisloPeriodika
-
n3:dodaniDat
n6:2005
n3:domaciTvurceVysledku
n12:1927469 n12:8764158 n12:2062402
n3:druhVysledku
n13:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
563015
n3:idVysledku
RIV/61388971:_____/04:00107943
n3:jazykVysledku
n11:eng
n3:klicovaSlova
ac-amp; wga
n3:klicoveSlovo
n10:wga n10:ac-amp
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[2FDD2A862661]
n3:nazevZdroje
Ornical Biomology Chemical
n3:obor
n4:EE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
6
n3:projekt
n8:OC%20D25.002 n8:GA203%2F04%2F1045
n3:rokUplatneniVysledku
n6:2004
n3:svazekPeriodika
2
n3:tvurceVysledku
Canada, F. J. Jiménez-Barbero, J. Křen, Vladimír Kuzma, Marek Aboitiz, N. Hušáková, Lucie
s:issn
1477-0520
s:numberOfPages
8