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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F14%3A00436098%21RIV15-GA0-61388963
rdf:type
n14:Vysledek skos:Concept
dcterms:description
Vacuolar hydrolases have been thoroughly characterized in Saccharomyces cerevisiae, but their homologues in the fungal pathogen Candida albicans have received less attention. The genes APR1 and CPY1 of C. albicans encode putative vacuolar aspartic proteinase and serine carboxypeptidase, respectively. We examined properties of apr1 Delta and cpy1 Delta mutants, showing that Cpy1p molecular species detected in cell lysates of apr1 Delta and its parental strain did not differ in molar mass. Processing of Cpy1p precursor is apparently independent of Apr1p. This is in contrast to S. cerevisiae, where vacuolar aspartic proteinase Pep4p is known to participate in the activation of other vacuolar hydrolases including serine carboxypeptidase. We also found that both apr1 Delta and cpy1 Delta strains are able to form hyphae in nutrient-rich filamentation media. However, proline as a sole nitrogen source induced filamentation only in cpy1 Delta and its parental strain, but not in apr1 Delta. This indicates the importance of Apr1p for the morphological transition under nitrogen-limited conditions. Despite that, the ability of apr1 Delta to kill murine macrophages was not reduced under the conditions tested. Vacuolar hydrolases have been thoroughly characterized in Saccharomyces cerevisiae, but their homologues in the fungal pathogen Candida albicans have received less attention. The genes APR1 and CPY1 of C. albicans encode putative vacuolar aspartic proteinase and serine carboxypeptidase, respectively. We examined properties of apr1 Delta and cpy1 Delta mutants, showing that Cpy1p molecular species detected in cell lysates of apr1 Delta and its parental strain did not differ in molar mass. Processing of Cpy1p precursor is apparently independent of Apr1p. This is in contrast to S. cerevisiae, where vacuolar aspartic proteinase Pep4p is known to participate in the activation of other vacuolar hydrolases including serine carboxypeptidase. We also found that both apr1 Delta and cpy1 Delta strains are able to form hyphae in nutrient-rich filamentation media. However, proline as a sole nitrogen source induced filamentation only in cpy1 Delta and its parental strain, but not in apr1 Delta. This indicates the importance of Apr1p for the morphological transition under nitrogen-limited conditions. Despite that, the ability of apr1 Delta to kill murine macrophages was not reduced under the conditions tested.
dcterms:title
Intracellular aspartic proteinase Apr1p of Candida albicans is required for morphological transition under nitrogen-limited conditions but not for macrophage killing Intracellular aspartic proteinase Apr1p of Candida albicans is required for morphological transition under nitrogen-limited conditions but not for macrophage killing
skos:prefLabel
Intracellular aspartic proteinase Apr1p of Candida albicans is required for morphological transition under nitrogen-limited conditions but not for macrophage killing Intracellular aspartic proteinase Apr1p of Candida albicans is required for morphological transition under nitrogen-limited conditions but not for macrophage killing
skos:notation
RIV/61388963:_____/14:00436098!RIV15-GA0-61388963
n3:aktivita
n16:P n16:I
n3:aktivity
I, P(GA310/09/1945), P(LO1302)
n3:cisloPeriodika
6
n3:dodaniDat
n8:2015
n3:domaciTvurceVysledku
n7:8255946 n7:5300169 n7:6949924 n7:2264102
n3:druhVysledku
n17:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
22594
n3:idVysledku
RIV/61388963:_____/14:00436098
n3:jazykVysledku
n13:eng
n3:klicovaSlova
Candida albicans; yeast vacuole lysosome; Saccharomyces cerevisiae
n3:klicoveSlovo
n11:Candida%20albicans n11:yeast%20vacuole%20lysosome n11:Saccharomyces%20cerevisiae
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[C2C09061383F]
n3:nazevZdroje
Folia Microbiologica
n3:obor
n10:EE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n4:GA310%2F09%2F1945 n4:LO1302
n3:rokUplatneniVysledku
n8:2014
n3:svazekPeriodika
59
n3:tvurceVysledku
Hrušková-Heidingsfeldová, Olga Hájek, Miroslav Pichová, Iva Bauerová, Václava
n3:wos
000343812600005
s:issn
0015-5632
s:numberOfPages
9
n18:doi
10.1007/s12223-014-0324-4