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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F14%3A00427919%21RIV15-TA0-61388963
rdf:type
skos:Concept n19:Vysledek
rdfs:seeAlso
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0093322
dcterms:description
Numerous Delta 12-, Delta 15- and multifunctional membrane fatty acid desaturases (FADs) have been identified in fungi, revealing great variability in the enzymatic specificities of FADs involved in biosynthesis of polyunsaturated fatty acids (PUFAs). Here, we report gene isolation and characterization of novel Delta 12/Delta 15- and Delta 15-FADs named CpFad2 and CpFad3, respectively, from the opportunistic pathogenic yeast Candida parapsilosis. Overexpression of CpFad3 in Saccharomyces cerevisiae strains supplemented with linoleic acid (Delta 9,Delta 12-18:2) and hexadecadienoic acid (Delta 9,Delta 12-16:2) leads to accumulation of Delta 15-PUFAs, i.e., alpha-linolenic acid (Delta 9,Delta 12,Delta 15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (Delta 9,Delta 12,Delta 15-16:3). CpFad2 produces a range of Delta 12- and Delta 15-PUFAs. The major products of CpFad2 are linoleic and hexadecadienoic acid (Delta 9,Delta 12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (Delta 9,Delta 12,Delta 15-16:3). Using GC/MS analysis of trimethylsilyl derivatives, we identified ricinoleic acid (12-hydroxy-9-octadecenoic acid) as an additional product of CpFad2. These results demonstrate that CpFAD2 is a multifunctional FAD and indicate that detailed analysis of fatty acid derivatives might uncover a range of enzymatic selectivities in other Delta 12-FADs from budding yeasts (Ascomycota: Saccharomycotina). Numerous Delta 12-, Delta 15- and multifunctional membrane fatty acid desaturases (FADs) have been identified in fungi, revealing great variability in the enzymatic specificities of FADs involved in biosynthesis of polyunsaturated fatty acids (PUFAs). Here, we report gene isolation and characterization of novel Delta 12/Delta 15- and Delta 15-FADs named CpFad2 and CpFad3, respectively, from the opportunistic pathogenic yeast Candida parapsilosis. Overexpression of CpFad3 in Saccharomyces cerevisiae strains supplemented with linoleic acid (Delta 9,Delta 12-18:2) and hexadecadienoic acid (Delta 9,Delta 12-16:2) leads to accumulation of Delta 15-PUFAs, i.e., alpha-linolenic acid (Delta 9,Delta 12,Delta 15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (Delta 9,Delta 12,Delta 15-16:3). CpFad2 produces a range of Delta 12- and Delta 15-PUFAs. The major products of CpFad2 are linoleic and hexadecadienoic acid (Delta 9,Delta 12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (Delta 9,Delta 12,Delta 15-16:3). Using GC/MS analysis of trimethylsilyl derivatives, we identified ricinoleic acid (12-hydroxy-9-octadecenoic acid) as an additional product of CpFad2. These results demonstrate that CpFAD2 is a multifunctional FAD and indicate that detailed analysis of fatty acid derivatives might uncover a range of enzymatic selectivities in other Delta 12-FADs from budding yeasts (Ascomycota: Saccharomycotina).
dcterms:title
Delta 12-Fatty Acid Desaturase from Candida parapsilosis Is a Multifunctional Desaturase Producing a Range of Polyunsaturated and Hydroxylated Fatty Acids Delta 12-Fatty Acid Desaturase from Candida parapsilosis Is a Multifunctional Desaturase Producing a Range of Polyunsaturated and Hydroxylated Fatty Acids
skos:prefLabel
Delta 12-Fatty Acid Desaturase from Candida parapsilosis Is a Multifunctional Desaturase Producing a Range of Polyunsaturated and Hydroxylated Fatty Acids Delta 12-Fatty Acid Desaturase from Candida parapsilosis Is a Multifunctional Desaturase Producing a Range of Polyunsaturated and Hydroxylated Fatty Acids
skos:notation
RIV/61388963:_____/14:00427919!RIV15-TA0-61388963
n4:aktivita
n7:P n7:I
n4:aktivity
I, P(TA01011461)
n4:cisloPeriodika
3
n4:dodaniDat
n11:2015
n4:domaciTvurceVysledku
n14:1935240 n14:7625502 n14:6949924 n14:2264102
n4:druhVysledku
n6:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n9:predkladatel
n4:idSjednocenehoVysledku
10149
n4:idVysledku
RIV/61388963:_____/14:00427919
n4:jazykVysledku
n12:eng
n4:klicovaSlova
yeast Saccharomyces cerevisiae; omega-3 fatty acid desaturase; heterologous expression
n4:klicoveSlovo
n5:yeast%20Saccharomyces%20cerevisiae n5:omega-3%20fatty%20acid%20desaturase n5:heterologous%20expression
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[5541524C4F3B]
n4:nazevZdroje
PLoS ONE
n4:obor
n15:CE
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
5
n4:projekt
n18:TA01011461
n4:rokUplatneniVysledku
n11:2014
n4:svazekPeriodika
9
n4:tvurceVysledku
Hrušková-Heidingsfeldová, Olga Buček, Aleš Pichová, Iva Matoušková, Petra Sychrová, Hana
n4:wos
000333678100043
s:issn
1932-6203
s:numberOfPages
10
n8:doi
10.1371/journal.pone.0093322