This HTML5 document contains 44 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n16http://linked.opendata.cz/resource/domain/vavai/projekt/
n13http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n4http://linked.opendata.cz/resource/domain/vavai/subjekt/
n3http://linked.opendata.cz/ontology/domain/vavai/
n19http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F61388963%3A_____%2F12%3A00377953%21RIV13-AV0-61388963/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n5http://linked.opendata.cz/ontology/domain/vavai/riv/
n8http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n6http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n18http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n11http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n7http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n10http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F12%3A00377953%21RIV13-AV0-61388963
rdf:type
n3:Vysledek skos:Concept
dcterms:description
A method for enzymatic production of short (10-20 nt) cytosine-modified oligonucleotides was developed by nicking enzyme amplification reaction using Vent(exo-) polymerase, Nt.BstNBI nicking endonuclease and 5-substituted dCTP derivatives. The methodology including isolation was scaled up to nanomolar amounts and was proved to be suitable for production of diverse base-modified short single-stranded oligonucleotides (inaccessible by other enzymatic methods) that are of potential interest as labelled primers or functionalized aptamers. A method for enzymatic production of short (10-20 nt) cytosine-modified oligonucleotides was developed by nicking enzyme amplification reaction using Vent(exo-) polymerase, Nt.BstNBI nicking endonuclease and 5-substituted dCTP derivatives. The methodology including isolation was scaled up to nanomolar amounts and was proved to be suitable for production of diverse base-modified short single-stranded oligonucleotides (inaccessible by other enzymatic methods) that are of potential interest as labelled primers or functionalized aptamers.
dcterms:title
Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction
skos:prefLabel
Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction
skos:notation
RIV/61388963:_____/12:00377953!RIV13-AV0-61388963
n3:predkladatel
n4:ico%3A61388963
n5:aktivita
n18:I n18:P
n5:aktivity
I, P(GA203/09/0317)
n5:cisloPeriodika
55
n5:dodaniDat
n10:2013
n5:domaciTvurceVysledku
n13:3547930 n13:6659160
n5:druhVysledku
n17:J
n5:duvernostUdaju
n14:S
n5:entitaPredkladatele
n19:predkladatel
n5:idSjednocenehoVysledku
161362
n5:idVysledku
RIV/61388963:_____/12:00377953
n5:jazykVysledku
n11:eng
n5:klicovaSlova
cross-coupling reactions; nucleoside triphosphates; functionalized DNA; restriction endonucleases; polymerase incorporation
n5:klicoveSlovo
n6:nucleoside%20triphosphates n6:functionalized%20DNA n6:polymerase%20incorporation n6:cross-coupling%20reactions n6:restriction%20endonucleases
n5:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n5:kontrolniKodProRIV
[8E3AE809B893]
n5:nazevZdroje
Chemical Communications
n5:obor
n7:CC
n5:pocetDomacichTvurcuVysledku
2
n5:pocetTvurcuVysledku
2
n5:projekt
n16:GA203%2F09%2F0317
n5:rokUplatneniVysledku
n10:2012
n5:svazekPeriodika
48
n5:tvurceVysledku
Hocek, Michal Ménová, Petra
n5:wos
000305255300010
s:issn
1359-7345
s:numberOfPages
3
n8:doi
10.1039/c2cc32930a