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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F12%3A00377777%21RIV13-AV0-61388963
rdf:type
skos:Concept n10:Vysledek
dcterms:description
5-(5-Formylthienyl)-, 5-(4-formylphenyl)- and 5-(2-fluoro-5-formylphenyl)cytosine 2’-deoxyribonucleoside mono- and triphosphates were prepared by aqueous Suzuki–Miyaura cross-coupling of 5-iodocytosine nucleotides with the corresponding formylarylboronic acids. The modified dCTPs were excellent substrates for DNA polymerases and were incorporated into DNA by primer extension or PCR. Reductive aminations of the model nucleotides with lysine or lysine-containing tripeptide were studied and optimized. In aqueous phosphate buffer (pH 6.7) the yields of the reductive aminations with tripeptide were up to 25%. Bioconjugation of an aldehyde-containing DNA with a lysine-containing tripeptide was achieved through reductive amination in yields of up to 90% in aqueous phosphate buffer. 5-(5-Formylthienyl)-, 5-(4-formylphenyl)- and 5-(2-fluoro-5-formylphenyl)cytosine 2’-deoxyribonucleoside mono- and triphosphates were prepared by aqueous Suzuki–Miyaura cross-coupling of 5-iodocytosine nucleotides with the corresponding formylarylboronic acids. The modified dCTPs were excellent substrates for DNA polymerases and were incorporated into DNA by primer extension or PCR. Reductive aminations of the model nucleotides with lysine or lysine-containing tripeptide were studied and optimized. In aqueous phosphate buffer (pH 6.7) the yields of the reductive aminations with tripeptide were up to 25%. Bioconjugation of an aldehyde-containing DNA with a lysine-containing tripeptide was achieved through reductive amination in yields of up to 90% in aqueous phosphate buffer.
dcterms:title
Synthesis of Aldehyde-Linked Nucleotides and DNA and Their Bioconjugations with Lysine and Peptides through Reductive Amination Synthesis of Aldehyde-Linked Nucleotides and DNA and Their Bioconjugations with Lysine and Peptides through Reductive Amination
skos:prefLabel
Synthesis of Aldehyde-Linked Nucleotides and DNA and Their Bioconjugations with Lysine and Peptides through Reductive Amination Synthesis of Aldehyde-Linked Nucleotides and DNA and Their Bioconjugations with Lysine and Peptides through Reductive Amination
skos:notation
RIV/61388963:_____/12:00377777!RIV13-AV0-61388963
n10:predkladatel
n15:ico%3A61388963
n3:aktivita
n5:P n5:I n5:Z
n3:aktivity
I, P(GA203/09/0317), Z(AV0Z40550506)
n3:cisloPeriodika
13
n3:dodaniDat
n6:2013
n3:domaciTvurceVysledku
n16:6659160 n16:3603652 n16:5468442
n3:druhVysledku
n20:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
172951
n3:idVysledku
RIV/61388963:_____/12:00377777
n3:jazykVysledku
n8:eng
n3:klicovaSlova
nucleotides; aldehydes; DNA; reductive amination; bioconjugations
n3:klicoveSlovo
n7:nucleotides n7:reductive%20amination n7:bioconjugations n7:DNA n7:aldehydes
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[E495D93D1BBA]
n3:nazevZdroje
Chemistry - A European Journal
n3:obor
n11:CC
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n12:GA203%2F09%2F0317
n3:rokUplatneniVysledku
n6:2012
n3:svazekPeriodika
18
n3:tvurceVysledku
Raindlová, Veronika Pohl, Radek Hocek, Michal
n3:wos
000301775300033
n3:zamer
n18:AV0Z40550506
s:issn
0947-6539
s:numberOfPages
8
n14:doi
10.1002/chem.201103270