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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F12%3A00377722%21RIV13-AV0-61388963
rdf:type
n11:Vysledek skos:Concept
dcterms:description
The design of four new fluorinated biaryl fluorescent labels and their attachment to nucleosides and nucleoside triphosphates (dNTPs) by the aqueous cross-coupling reactions of biarylboronates is reported. The modified dNTPs were good substrates for KOD XL polymerase and were enzymatically incorporated into DNA probes. The photophysical properties of the biaryl-modified nucleosides, dNTPs, and DNA were studied systematically. Some labels were used for detection of secondary structure changes, mismatch pairing or pH microenvironment. The design of four new fluorinated biaryl fluorescent labels and their attachment to nucleosides and nucleoside triphosphates (dNTPs) by the aqueous cross-coupling reactions of biarylboronates is reported. The modified dNTPs were good substrates for KOD XL polymerase and were enzymatically incorporated into DNA probes. The photophysical properties of the biaryl-modified nucleosides, dNTPs, and DNA were studied systematically. Some labels were used for detection of secondary structure changes, mismatch pairing or pH microenvironment.
dcterms:title
Synthesis and Photophysical Properties of Biaryl-Substituted Nucleos(t)ides. Polymerase Synthesis of DNA Probes Bearing Solvatochromic and pH Sensitive Dual Fluorescent and 19F NMR Labels Synthesis and Photophysical Properties of Biaryl-Substituted Nucleos(t)ides. Polymerase Synthesis of DNA Probes Bearing Solvatochromic and pH Sensitive Dual Fluorescent and 19F NMR Labels
skos:prefLabel
Synthesis and Photophysical Properties of Biaryl-Substituted Nucleos(t)ides. Polymerase Synthesis of DNA Probes Bearing Solvatochromic and pH Sensitive Dual Fluorescent and 19F NMR Labels Synthesis and Photophysical Properties of Biaryl-Substituted Nucleos(t)ides. Polymerase Synthesis of DNA Probes Bearing Solvatochromic and pH Sensitive Dual Fluorescent and 19F NMR Labels
skos:notation
RIV/61388963:_____/12:00377722!RIV13-AV0-61388963
n11:predkladatel
n15:ico%3A61388963
n3:aktivita
n5:Z n5:P
n3:aktivity
P(GA203/09/0317), Z(AV0Z40550506)
n3:cisloPeriodika
2
n3:dodaniDat
n12:2013
n3:domaciTvurceVysledku
n14:7465157 n14:6614655 n14:6659160 n14:3603652
n3:druhVysledku
n17:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
172938
n3:idVysledku
RIV/61388963:_____/12:00377722
n3:jazykVysledku
n9:eng
n3:klicovaSlova
nucleotides; biaryls; fluorescence; DNA
n3:klicoveSlovo
n4:biaryls n4:fluorescence n4:DNA n4:nucleotides
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[38F846F488DD]
n3:nazevZdroje
Journal of Organic Chemistry
n3:obor
n20:CC
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n18:GA203%2F09%2F0317
n3:rokUplatneniVysledku
n12:2012
n3:svazekPeriodika
77
n3:tvurceVysledku
Hocek, Michal Riedl, Jan Pohl, Radek Rulíšek, Lubomír
n3:wos
000299238400024
n3:zamer
n10:AV0Z40550506
s:issn
0022-3263
s:numberOfPages
19
n16:doi
10.1021/jo202321g