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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F12%3A00376450%21RIV13-AV0-61388963
rdf:type
n16:Vysledek skos:Concept
rdfs:seeAlso
http://www.biomedcentral.com/1471-2091/13/3
dcterms:description
We describe biochemical and immunological properties of the native alpha-amylase allergen Aca s 4 from Acarus siro, a medically important storage mite. A. siro produced a high level of alpha-amylase activity attributed to Aca s 4. This enzyme was purified and identified by protein sequencing and LC-MS/MS analysis. Aca s 4 showed a distinct inhibition pattern and an unusual alpha-amylolytic activity with low sensitivity to activation by chloride ions. Homology modeling of Aca s 4 revealed a structural change in the chloride-binding site that may account for this activation pattern. Aca s 4 was recognized by IgE from house dust mite-sensitive patients, and potential epitopes for cross-reactivity with house dust mite group 4 allergens were found. We present the first protein-level characterization of a group 4 allergen from storage mites. Due to its high production and IgE reactivity, Aca s 4 is potentially relevant to allergic hypersensitivity. We describe biochemical and immunological properties of the native alpha-amylase allergen Aca s 4 from Acarus siro, a medically important storage mite. A. siro produced a high level of alpha-amylase activity attributed to Aca s 4. This enzyme was purified and identified by protein sequencing and LC-MS/MS analysis. Aca s 4 showed a distinct inhibition pattern and an unusual alpha-amylolytic activity with low sensitivity to activation by chloride ions. Homology modeling of Aca s 4 revealed a structural change in the chloride-binding site that may account for this activation pattern. Aca s 4 was recognized by IgE from house dust mite-sensitive patients, and potential epitopes for cross-reactivity with house dust mite group 4 allergens were found. We present the first protein-level characterization of a group 4 allergen from storage mites. Due to its high production and IgE reactivity, Aca s 4 is potentially relevant to allergic hypersensitivity.
dcterms:title
Enzymatic activity and immunoreactivity of Aca s 4, an alpha-amylase allergen from the storage mite Acarus siro Enzymatic activity and immunoreactivity of Aca s 4, an alpha-amylase allergen from the storage mite Acarus siro
skos:prefLabel
Enzymatic activity and immunoreactivity of Aca s 4, an alpha-amylase allergen from the storage mite Acarus siro Enzymatic activity and immunoreactivity of Aca s 4, an alpha-amylase allergen from the storage mite Acarus siro
skos:notation
RIV/61388963:_____/12:00376450!RIV13-AV0-61388963
n16:predkladatel
n19:ico%3A61388963
n4:aktivita
n11:P n11:Z
n4:aktivity
P(GP525/09/P600), Z(AV0Z40550506)
n4:cisloPeriodika
3
n4:dodaniDat
n12:2013
n4:domaciTvurceVysledku
n5:8110816 n5:8133859 n5:2396955 n5:5796644 n5:7667108 n5:6413269
n4:druhVysledku
n6:J
n4:duvernostUdaju
n21:S
n4:entitaPredkladatele
n14:predkladatel
n4:idSjednocenehoVysledku
134574
n4:idVysledku
RIV/61388963:_____/12:00376450
n4:jazykVysledku
n13:eng
n4:klicovaSlova
Aca s 4; Acarus siro; alpha-amylases
n4:klicoveSlovo
n7:Aca%20s%204 n7:Acarus%20siro n7:alpha-amylases
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[50ED5644D075]
n4:nazevZdroje
BMC Biochemistry
n4:obor
n15:CE
n4:pocetDomacichTvurcuVysledku
6
n4:pocetTvurcuVysledku
6
n4:projekt
n9:GP525%2F09%2FP600
n4:rokUplatneniVysledku
n12:2012
n4:svazekPeriodika
13
n4:tvurceVysledku
Marešová, Lucie Pytelková, Jana Šanda, Miloslav Lepšík, Martin Talacko, Pavel Mareš, Michael
n4:wos
000301863300001
n4:zamer
n10:AV0Z40550506
s:issn
1471-2091
s:numberOfPages
8
n18:doi
10.1186/1471-2091-13-3