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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F12%3A00376236%21RIV13-AV0-61388963
rdf:type
n4:Vysledek skos:Concept
dcterms:description
Methionyl aminopeptidases (MetAPs) are metallo-dependent proteases responsible for removing of N-terminal methionine residue of peptides and proteins during protein maturation and activation. In this report we use a comprehensive strategy to screen the substrate specificity of three methionyl aminopeptidases: Homo sapiens MetAP-1, Homo sapiens MetAP-2 and Escherichia coli MetAP-1. By utilizing a 65-membered fluorogenic substrate library consisting of natural and unnatural amino acids we established detailed substrate preferences of each enzyme in the S1 pocket. Our results show that this pocket is highly conserved for all investigated MetAPs, very stringent for methionine, and that several unnatural amino acids with methionine-like characteristics were also well hydrolyzed by MetAPs. The substrate-derived results were verified using several phosphonate and phosphinate-based inhibitors. Methionyl aminopeptidases (MetAPs) are metallo-dependent proteases responsible for removing of N-terminal methionine residue of peptides and proteins during protein maturation and activation. In this report we use a comprehensive strategy to screen the substrate specificity of three methionyl aminopeptidases: Homo sapiens MetAP-1, Homo sapiens MetAP-2 and Escherichia coli MetAP-1. By utilizing a 65-membered fluorogenic substrate library consisting of natural and unnatural amino acids we established detailed substrate preferences of each enzyme in the S1 pocket. Our results show that this pocket is highly conserved for all investigated MetAPs, very stringent for methionine, and that several unnatural amino acids with methionine-like characteristics were also well hydrolyzed by MetAPs. The substrate-derived results were verified using several phosphonate and phosphinate-based inhibitors.
dcterms:title
S1 pocket fingerprints of human and bacterial methionine aminopeptidases determined using fluorogenic libraries of substrates and phosphorus based inhibitors S1 pocket fingerprints of human and bacterial methionine aminopeptidases determined using fluorogenic libraries of substrates and phosphorus based inhibitors
skos:prefLabel
S1 pocket fingerprints of human and bacterial methionine aminopeptidases determined using fluorogenic libraries of substrates and phosphorus based inhibitors S1 pocket fingerprints of human and bacterial methionine aminopeptidases determined using fluorogenic libraries of substrates and phosphorus based inhibitors
skos:notation
RIV/61388963:_____/12:00376236!RIV13-AV0-61388963
n4:predkladatel
n5:ico%3A61388963
n3:aktivita
n12:P n12:Z
n3:aktivity
P(LC06077), Z(AV0Z40550506)
n3:cisloPeriodika
3
n3:dodaniDat
n20:2013
n3:domaciTvurceVysledku
n13:1606778 n13:3046486
n3:druhVysledku
n6:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
173182
n3:idVysledku
RIV/61388963:_____/12:00376236
n3:jazykVysledku
n7:eng
n3:klicovaSlova
methionine aminopeptidase; substrate library; protease; enzyme; inhibitor; substrate specificity
n3:klicoveSlovo
n11:protease n11:inhibitor n11:enzyme n11:methionine%20aminopeptidase n11:substrate%20specificity n11:substrate%20library
n3:kodStatuVydavatele
FR - Francouzská republika
n3:kontrolniKodProRIV
[90315ADE04DE]
n3:nazevZdroje
Biochimie
n3:obor
n15:CC
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
8
n3:projekt
n16:LC06077
n3:rokUplatneniVysledku
n20:2012
n3:svazekPeriodika
94
n3:tvurceVysledku
Salvesen, G. S. Gajda, A. Klein, Ch. D. Pícha, Jan Marschner, A. Drag, M. Poreba, M. Jiráček, Jiří
n3:wos
000301332200014
n3:zamer
n19:AV0Z40550506
s:issn
0300-9084
s:numberOfPages
7
n9:doi
10.1016/j.biochi.2011.10.014