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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F11%3A00389610%21RIV13-AV0-61388963
rdf:type
n9:Vysledek skos:Concept
dcterms:description
Regulation of biomineralization processes are mediated by extracellular matrix proteins that often exhibit proline-rich regions. Advanced bioinformatic methods were used to design the structure of artificial peptides (P1, P2, P3) based on proline-rich domains of extracellular proteins. Their effect on osteoblast differentiation and in vitro biomineralization was tested on MC3T3-E1 and human umbilical cord mesenchymal stem cells (hUCMSCs) and compared to the commercially available enamel matrix derivative (EMD). MC3T3-E1 and hUCMSCs treated with the synthetic peptides showed a decreased cytotoxicity after 24-48 h of treatment compared to control. MC373-E1 cells treated with EMD showed lower expression of osteoblast markers genes than cells treated with P2, except for collagen type I. In hUCMSCs, OC gene expression was higher in P2-treated cells compared to those treated with EMD or control. ALP activity was markedly increased in MC3T3-E1 cells incubated with P2 compared to other treatments. Similar results were observed in hUCMSCs. Further, P2 increased calcium deposition rate compared to EMD or control either in MC3T3-E1 or hUCMSCs. The observed effects of proline-rich peptides hold potential for both clinical applications and as a research tool in further investigations of the molecular basis of induced osteogenic cell differentiation. Regulation of biomineralization processes are mediated by extracellular matrix proteins that often exhibit proline-rich regions. Advanced bioinformatic methods were used to design the structure of artificial peptides (P1, P2, P3) based on proline-rich domains of extracellular proteins. Their effect on osteoblast differentiation and in vitro biomineralization was tested on MC3T3-E1 and human umbilical cord mesenchymal stem cells (hUCMSCs) and compared to the commercially available enamel matrix derivative (EMD). MC3T3-E1 and hUCMSCs treated with the synthetic peptides showed a decreased cytotoxicity after 24-48 h of treatment compared to control. MC373-E1 cells treated with EMD showed lower expression of osteoblast markers genes than cells treated with P2, except for collagen type I. In hUCMSCs, OC gene expression was higher in P2-treated cells compared to those treated with EMD or control. ALP activity was markedly increased in MC3T3-E1 cells incubated with P2 compared to other treatments. Similar results were observed in hUCMSCs. Further, P2 increased calcium deposition rate compared to EMD or control either in MC3T3-E1 or hUCMSCs. The observed effects of proline-rich peptides hold potential for both clinical applications and as a research tool in further investigations of the molecular basis of induced osteogenic cell differentiation.
dcterms:title
Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells
skos:prefLabel
Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells
skos:notation
RIV/61388963:_____/11:00389610!RIV13-AV0-61388963
n9:predkladatel
n11:ico%3A61388963
n3:aktivita
n17:Z
n3:aktivity
Z(AV0Z40550506)
n3:cisloPeriodika
2
n3:dodaniDat
n7:2013
n3:domaciTvurceVysledku
n16:7358946
n3:druhVysledku
n4:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
233939
n3:idVysledku
RIV/61388963:_____/11:00389610
n3:jazykVysledku
n12:eng
n3:klicovaSlova
proline-rich regions; synthetic peptides; bone formation; mineralization; In Vitro
n3:klicoveSlovo
n10:mineralization n10:synthetic%20peptides n10:In%20Vitro n10:proline-rich%20regions n10:bone%20formation
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[27690922EFA1]
n3:nazevZdroje
Journal of Biomaterials and Tissue Engineering
n3:obor
n15:EI
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n7:2011
n3:svazekPeriodika
1
n3:tvurceVysledku
Lyngstadaas, S. P. Vondrášek, Jiří Monjo, M. Gaya, A. Ramis, J. M. Rubert, M.
n3:wos
000312570600009
n3:zamer
n19:AV0Z40550506
s:issn
2157-9083
s:numberOfPages
12
n5:doi
10.1166/jbt.2011.1018