This HTML5 document contains 52 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n11http://linked.opendata.cz/resource/domain/vavai/projekt/
n7http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n13http://linked.opendata.cz/resource/domain/vavai/subjekt/
n12http://linked.opendata.cz/ontology/domain/vavai/
n17http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n16http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F61388963%3A_____%2F11%3A00370762%21RIV12-AV0-61388963/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n15http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n10http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n18http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n19http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n14http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F11%3A00370762%21RIV12-AV0-61388963
rdf:type
skos:Concept n12:Vysledek
dcterms:description
Electrochemical detection of DNA damage in vitro has been used to evaluate the antioxidant properties of transgenic tobacco plant extracts containing metallothionein (MT). This method is based on assessment of efficiency with which the studied plant extracts are able to reduce to the amount of damage of standard chicken DNA by free OH radicals prepared by the Fenton reaction. We have tested the MT isolated from rabbit liver as a standard reference solution and compared its activity with extracts from wild type tobacco plants, and from twelve clones of transgenic tobacco plants variously carrying human or yeast MT. The present experimental results clearly demonstrate that insertion of a MT gene into the DNA of tobacco plants significantly improves their antioxidant properties in comparison with the non transgenic plants. The method developed for the purpose of this study has been demonstrated to be suitable for rapid screening of various plants with respect to their potential for phytoremediation of heavy metals. Electrochemical detection of DNA damage in vitro has been used to evaluate the antioxidant properties of transgenic tobacco plant extracts containing metallothionein (MT). This method is based on assessment of efficiency with which the studied plant extracts are able to reduce to the amount of damage of standard chicken DNA by free OH radicals prepared by the Fenton reaction. We have tested the MT isolated from rabbit liver as a standard reference solution and compared its activity with extracts from wild type tobacco plants, and from twelve clones of transgenic tobacco plants variously carrying human or yeast MT. The present experimental results clearly demonstrate that insertion of a MT gene into the DNA of tobacco plants significantly improves their antioxidant properties in comparison with the non transgenic plants. The method developed for the purpose of this study has been demonstrated to be suitable for rapid screening of various plants with respect to their potential for phytoremediation of heavy metals.
dcterms:title
Investigation of the Antioxidant Properties of Metallothionein in Transgenic Tobacco Plants using Voltammetry at a Carbon Paste Electrode Investigation of the Antioxidant Properties of Metallothionein in Transgenic Tobacco Plants using Voltammetry at a Carbon Paste Electrode
skos:prefLabel
Investigation of the Antioxidant Properties of Metallothionein in Transgenic Tobacco Plants using Voltammetry at a Carbon Paste Electrode Investigation of the Antioxidant Properties of Metallothionein in Transgenic Tobacco Plants using Voltammetry at a Carbon Paste Electrode
skos:notation
RIV/61388963:_____/11:00370762!RIV12-AV0-61388963
n12:predkladatel
n13:ico%3A61388963
n3:aktivita
n4:P n4:Z
n3:aktivity
P(ED1.1.00/02.0068), P(GA102/08/1546), P(GA522/07/0692), Z(AV0Z40550506)
n3:cisloPeriodika
7
n3:dodaniDat
n14:2012
n3:domaciTvurceVysledku
n7:9344721
n3:druhVysledku
n19:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
205728
n3:idVysledku
RIV/61388963:_____/11:00370762
n3:jazykVysledku
n18:eng
n3:klicovaSlova
square wave voltammetry; carbon paste electrode; DNA; metallothionein
n3:klicoveSlovo
n15:metallothionein n15:DNA n15:carbon%20paste%20electrode n15:square%20wave%20voltammetry
n3:kodStatuVydavatele
CS - Státní svaz Srbska a Černé Hory
n3:kontrolniKodProRIV
[ABA3A04C9976]
n3:nazevZdroje
International Journal of Electrochemical Science
n3:obor
n9:CG
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
10
n3:projekt
n11:ED1.1.00%2F02.0068 n11:GA102%2F08%2F1546 n11:GA522%2F07%2F0692
n3:rokUplatneniVysledku
n14:2011
n3:svazekPeriodika
6
n3:tvurceVysledku
Hubálek, J. Prášek, J. Havel, L. Kižek, R. Shetivska, V. Adam, V. Macek, Tomáš Dioplan, V. Macková, M. Zehnálek, J.
n3:wos
000292331400042
n3:zamer
n17:AV0Z40550506
s:issn
1452-3981
s:numberOfPages
15