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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F11%3A00364215%21RIV12-AV0-61388963
rdf:type
n8:Vysledek skos:Concept
dcterms:description
The first transient chemical protection of DNA against RE cleavage was developed. (Triethylsilyl)ethynyl-modified 7-deaza-dATP is readily incorporated to DNA by primer extension or PCR using KOD XL polymerase. The silylethynyl-protected DNA resists the cleavage by restriction endonucleases (REs). After treatment with NH3, the deprotected DNA is fully cleavable by the REs. The first transient chemical protection of DNA against RE cleavage was developed. (Triethylsilyl)ethynyl-modified 7-deaza-dATP is readily incorporated to DNA by primer extension or PCR using KOD XL polymerase. The silylethynyl-protected DNA resists the cleavage by restriction endonucleases (REs). After treatment with NH3, the deprotected DNA is fully cleavable by the REs.
dcterms:title
Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases
skos:prefLabel
Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases
skos:notation
RIV/61388963:_____/11:00364215!RIV12-AV0-61388963
n8:predkladatel
n13:ico%3A61388963
n3:aktivita
n20:P n20:Z
n3:aktivity
P(GA203/09/0317), Z(AV0Z40550506)
n3:cisloPeriodika
37
n3:dodaniDat
n9:2012
n3:domaciTvurceVysledku
n7:2810824 n7:6659160 n7:3603652 n7:9618600
n3:druhVysledku
n17:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
235826
n3:idVysledku
RIV/61388963:_____/11:00364215
n3:jazykVysledku
n15:eng
n3:klicovaSlova
alkynes; DNA; protecting groups; nucleotides; restriction endonucleases
n3:klicoveSlovo
n4:protecting%20groups n4:nucleotides n4:restriction%20endonucleases n4:alkynes n4:DNA
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[E710560DDEE4]
n3:nazevZdroje
Angewandte Chemie. International Edition in English
n3:obor
n14:CC
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n16:GA203%2F09%2F0317
n3:rokUplatneniVysledku
n9:2011
n3:svazekPeriodika
50
n3:tvurceVysledku
Macíčková-Cahová, Hana Hocek, Michal Kielkowski, Pavel Pohl, Radek
n3:wos
000295261200055
n3:zamer
n19:AV0Z40550506
s:issn
1433-7851
s:numberOfPages
4
n12:doi
10.1002/anie.201102898