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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F11%3A00360994%21RIV12-AV0-61388963
rdf:type
skos:Concept n11:Vysledek
dcterms:description
Cytosin-5-yl ethynyl derivatives of pyrimidine and 7-deazaadenine 2-deoxyribonucleosides and nucleoside triphosphates (dNTPs) were prepared in one step by the aqueous Sonogashira coupling of unprotected halogenated nucleos(t)ides with 5-ethynylcytosine. The modified dNTPs were good substrates for DNA polymerases suitable for primer extension or PCR construction of DNA bearing acetylene-linked cytosine(s) in the major groove mimicking the flipped-out nucleotide. Cytosin-5-yl ethynyl derivatives of pyrimidine and 7-deazaadenine 2-deoxyribonucleosides and nucleoside triphosphates (dNTPs) were prepared in one step by the aqueous Sonogashira coupling of unprotected halogenated nucleos(t)ides with 5-ethynylcytosine. The modified dNTPs were good substrates for DNA polymerases suitable for primer extension or PCR construction of DNA bearing acetylene-linked cytosine(s) in the major groove mimicking the flipped-out nucleotide.
dcterms:title
Synthesis of acetylene linked double-nucleobase nucleos(t)ide building blocks and polymerase construction of DNA containing cytosines in the major groove Synthesis of acetylene linked double-nucleobase nucleos(t)ide building blocks and polymerase construction of DNA containing cytosines in the major groove
skos:prefLabel
Synthesis of acetylene linked double-nucleobase nucleos(t)ide building blocks and polymerase construction of DNA containing cytosines in the major groove Synthesis of acetylene linked double-nucleobase nucleos(t)ide building blocks and polymerase construction of DNA containing cytosines in the major groove
skos:notation
RIV/61388963:_____/11:00360994!RIV12-AV0-61388963
n11:predkladatel
n12:ico%3A61388963
n4:aktivita
n14:P n14:Z
n4:aktivity
P(GA203/09/0317), P(LC512), Z(AV0Z40550506)
n4:cisloPeriodika
9
n4:dodaniDat
n5:2012
n4:domaciTvurceVysledku
n10:9618600 n10:3603652 n10:6659160
n4:druhVysledku
n15:J
n4:duvernostUdaju
n20:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
233896
n4:idVysledku
RIV/61388963:_____/11:00360994
n4:jazykVysledku
n9:eng
n4:klicovaSlova
nucleoside triphosphates; functionalized DNA; cross-coupling reactions; nucleic-acids
n4:klicoveSlovo
n13:cross-coupling%20reactions n13:functionalized%20DNA n13:nucleic-acids n13:nucleoside%20triphosphates
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[967AF0A41396]
n4:nazevZdroje
Journal of Organic Chemistry
n4:obor
n6:CC
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
3
n4:projekt
n7:GA203%2F09%2F0317 n7:LC512
n4:rokUplatneniVysledku
n5:2011
n4:svazekPeriodika
76
n4:tvurceVysledku
Hocek, Michal Pohl, Radek Kielkowski, Pavel
n4:wos
000289956900051
n4:zamer
n8:AV0Z40550506
s:issn
0022-3263
s:numberOfPages
6
n19:doi
10.1021/jo200436j