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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F11%3A00359988%21RIV12-AV0-61388963
rdf:type
skos:Concept n6:Vysledek
dcterms:description
Two capillary electrophoretic methods were developed and evaluated for measurement of glycated hemoglobin HbA1c. Firstly, a capillary zone electrophoresis analysis is performed with a sodium tetraborate buffer of pH 9.3 as background electrolyte in a neutrally coated capillary. HbA1c is separated from HbA0 due to specific interactions of borate anions with the cis-diol pattern in the saccharide moiety of glycohemoglobin. Secondly, a capillary isoelectric focusing method, which exploits a difference in pI values of HbA0 and HbA1c, is performed with Servalyt pH 6-8 or alternatively with Biolyte pH 6-8 carrier ampholytes spiked with a narrow pH cut of pH 7.2 prepared by preparative fractionation of Servalyt pH 4-9 carrier ampholytes. Two capillary electrophoretic methods were developed and evaluated for measurement of glycated hemoglobin HbA1c. Firstly, a capillary zone electrophoresis analysis is performed with a sodium tetraborate buffer of pH 9.3 as background electrolyte in a neutrally coated capillary. HbA1c is separated from HbA0 due to specific interactions of borate anions with the cis-diol pattern in the saccharide moiety of glycohemoglobin. Secondly, a capillary isoelectric focusing method, which exploits a difference in pI values of HbA0 and HbA1c, is performed with Servalyt pH 6-8 or alternatively with Biolyte pH 6-8 carrier ampholytes spiked with a narrow pH cut of pH 7.2 prepared by preparative fractionation of Servalyt pH 4-9 carrier ampholytes.
dcterms:title
Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing
skos:prefLabel
Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing
skos:notation
RIV/61388963:_____/11:00359988!RIV12-AV0-61388963
n6:predkladatel
n7:ico%3A61388963
n3:aktivita
n8:P n8:Z
n3:aktivity
P(GA203/08/1428), P(GP203/09/P485), Z(AV0Z40550506)
n3:cisloPeriodika
1
n3:dodaniDat
n12:2012
n3:domaciTvurceVysledku
n14:7710070 n14:9379789
n3:druhVysledku
n19:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
185666
n3:idVysledku
RIV/61388963:_____/11:00359988
n3:jazykVysledku
n17:eng
n3:klicovaSlova
capillary zone electrophoresis; capillary isoelectric focusing; glycated hemoglobin HbA1c
n3:klicoveSlovo
n5:capillary%20zone%20electrophoresis n5:glycated%20hemoglobin%20HbA1c n5:capillary%20isoelectric%20focusing
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[A079EA3951E4]
n3:nazevZdroje
Analytical Biochemistry
n3:obor
n15:CB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n9:GA203%2F08%2F1428 n9:GP203%2F09%2FP485
n3:rokUplatneniVysledku
n12:2011
n3:svazekPeriodika
413
n3:tvurceVysledku
Koval, Dušan Kašička, Václav Cottet, H.
n3:wos
000289607900002
n3:zamer
n20:AV0Z40550506
s:issn
0003-2697
s:numberOfPages
8
n18:doi
10.1016/j.ab.2011.01.048