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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F10%3A00353703%21RIV11-AV0-61388963
rdf:type
skos:Concept n13:Vysledek
dcterms:description
A novel, efficient, two-step methodology is presented for construction of base-modified oligonucleotides or DNA involving aqueous cross-coupling reactions of halogenated nucleoside triphosphates (dNTPs) with terminal acetylenes or arylboronic acids, followed by polymerase incorporation of the modified dNTPs either using primer extension (PEX) or polymerase chain reaction (PCR). A novel, efficient, two-step methodology is presented for construction of base-modified oligonucleotides or DNA involving aqueous cross-coupling reactions of halogenated nucleoside triphosphates (dNTPs) with terminal acetylenes or arylboronic acids, followed by polymerase incorporation of the modified dNTPs either using primer extension (PEX) or polymerase chain reaction (PCR).
dcterms:title
Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA
skos:prefLabel
Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA
skos:notation
RIV/61388963:_____/10:00353703!RIV11-AV0-61388963
n4:aktivita
n11:Z
n4:aktivity
Z(AV0Z40550506)
n4:dodaniDat
n9:2011
n4:domaciTvurceVysledku
n10:9804668 n10:6659160 n10:2810824
n4:druhVysledku
n17:C
n4:duvernostUdaju
n8:S
n4:entitaPredkladatele
n16:predkladatel
n4:idSjednocenehoVysledku
252392
n4:idVysledku
RIV/61388963:_____/10:00353703
n4:jazykVysledku
n15:eng
n4:klicovaSlova
nucleoside triphosphates; DNA polymerase; cross-coupling; DNA conjugates
n4:klicoveSlovo
n6:DNA%20polymerase n6:nucleoside%20triphosphates n6:DNA%20conjugates n6:cross-coupling
n4:kontrolniKodProRIV
[FD829C54B154]
n4:mistoVydani
New York
n4:nazevZdroje
Current Protocols in Chemical Biology
n4:obor
n5:CC
n4:pocetDomacichTvurcuVysledku
3
n4:pocetStranKnihy
550
n4:pocetTvurcuVysledku
3
n4:rokUplatneniVysledku
n9:2010
n4:tvurceVysledku
Macíčková-Cahová, Hana Hocek, Michal Vrábel, Milan
n4:zamer
n14:AV0Z40550506
s:numberOfPages
14
n12:hasPublisher
Wiley Interscience
n18:isbn
9780470559277