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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F10%3A00343324%21RIV11-MSM-61388963
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Human serine racemase (hSR) is a cytosolic pyridoxal-5-phosphate dependent enzyme responsible for production of D-serine in the central nervous system. D-Serine acts as an endogenous coagonist of N-methyl-D-aspartate receptor ion channels. Increased levels of D-serine have been linked to amyotrophic lateral sclerosis and Alzheimer’s disease.Here, we present a strategy for the generation and screening of random hSR mutants. From a library of randomly mutated hSR variants, twenty-seven soluble mutants were selected, expressed, and evaluated for enzymatic activity. Taking three carefully characterized mutants as an example, we show how this strategy can be used to pinpoint structurally and functionally important residues. In particular, we identify S84 and P111 as residues crucial for hSR activity and C217 and K221 as residues important for binding of the Mg2+ cofactor. Human serine racemase (hSR) is a cytosolic pyridoxal-5-phosphate dependent enzyme responsible for production of D-serine in the central nervous system. D-Serine acts as an endogenous coagonist of N-methyl-D-aspartate receptor ion channels. Increased levels of D-serine have been linked to amyotrophic lateral sclerosis and Alzheimer’s disease.Here, we present a strategy for the generation and screening of random hSR mutants. From a library of randomly mutated hSR variants, twenty-seven soluble mutants were selected, expressed, and evaluated for enzymatic activity. Taking three carefully characterized mutants as an example, we show how this strategy can be used to pinpoint structurally and functionally important residues. In particular, we identify S84 and P111 as residues crucial for hSR activity and C217 and K221 as residues important for binding of the Mg2+ cofactor.
dcterms:title
Random mutagenesis of human serine racemase reveals residues important for the enzymatic activity Random mutagenesis of human serine racemase reveals residues important for the enzymatic activity
skos:prefLabel
Random mutagenesis of human serine racemase reveals residues important for the enzymatic activity Random mutagenesis of human serine racemase reveals residues important for the enzymatic activity
skos:notation
RIV/61388963:_____/10:00343324!RIV11-MSM-61388963
n3:aktivita
n5:Z n5:P
n3:aktivity
P(1M0508), Z(AV0Z40550506)
n3:cisloPeriodika
1
n3:dodaniDat
n13:2011
n3:domaciTvurceVysledku
n11:7120532 Hoffman, Hillary Elizabeth n11:8705275
n3:druhVysledku
n10:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
283875
n3:idVysledku
RIV/61388963:_____/10:00343324
n3:jazykVysledku
n16:eng
n3:klicovaSlova
D-serine; serine racemase; random mutagenesis
n3:klicoveSlovo
n4:D-serine n4:random%20mutagenesis n4:serine%20racemase
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[B22330C2580C]
n3:nazevZdroje
Collection of Czechoslovak Chemical Communications
n3:obor
n15:CE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n14:1M0508
n3:rokUplatneniVysledku
n13:2010
n3:svazekPeriodika
75
n3:tvurceVysledku
Konvalinka, Jan Hoffman, Hillary Elizabeth Jirásková, Jana Zvelebil, M.
n3:wos
000274668100004
n3:zamer
n9:AV0Z40550506
s:issn
0010-0765
s:numberOfPages
21