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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F10%3A00342374%21RIV11-GA0-61388963
rdf:type
skos:Concept n13:Vysledek
dcterms:description
Reactive aldehyde-modified DNA was prapered in two steps by cross-coupling reactions of halogenated dNTPs with 4-formylthiophene-2-boronic acid followed by polymerase (PEX or PCR) incorporations of the modified nucleotides to DNA. Aqueous hydrazone-formation was used in conjugation with aryl hydrazines for DNA staining. Reactive aldehyde-modified DNA was prapered in two steps by cross-coupling reactions of halogenated dNTPs with 4-formylthiophene-2-boronic acid followed by polymerase (PEX or PCR) incorporations of the modified nucleotides to DNA. Aqueous hydrazone-formation was used in conjugation with aryl hydrazines for DNA staining.
dcterms:title
Direct polymerase synthesis of reactive aldehyde-functionalized DNA and its conjugation and staining with hydrazines Direct polymerase synthesis of reactive aldehyde-functionalized DNA and its conjugation and staining with hydrazines
skos:prefLabel
Direct polymerase synthesis of reactive aldehyde-functionalized DNA and its conjugation and staining with hydrazines Direct polymerase synthesis of reactive aldehyde-functionalized DNA and its conjugation and staining with hydrazines
skos:notation
RIV/61388963:_____/10:00342374!RIV11-GA0-61388963
n4:aktivita
n11:Z n11:P
n4:aktivity
P(GA203/09/0317), P(LC512), Z(AV0Z40550506)
n4:cisloPeriodika
6
n4:dodaniDat
n10:2011
n4:domaciTvurceVysledku
n9:6659160 n9:3603652 n9:2396955 n9:5468442
n4:druhVysledku
n15:J
n4:duvernostUdaju
n12:S
n4:entitaPredkladatele
n16:predkladatel
n4:idSjednocenehoVysledku
254549
n4:idVysledku
RIV/61388963:_____/10:00342374
n4:jazykVysledku
n6:eng
n4:klicovaSlova
aldehydes; nucleotides; nucleoside triphosphates; DNA polymerase; DNA conjugates
n4:klicoveSlovo
n7:DNA%20polymerase n7:aldehydes n7:DNA%20conjugates n7:nucleotides n7:nucleoside%20triphosphates
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[A3CC7CAA6BD0]
n4:nazevZdroje
Angewandte Chemie. International Edition in English
n4:obor
n18:CC
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
4
n4:projekt
n14:LC512 n14:GA203%2F09%2F0317
n4:rokUplatneniVysledku
n10:2010
n4:svazekPeriodika
49
n4:tvurceVysledku
Raindlová, Veronika Pohl, Radek Šanda, Miloslav Hocek, Michal
n4:wos
000274571500008
n4:zamer
n17:AV0Z40550506
s:issn
1433-7851
s:numberOfPages
3