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Statements

Subject Item
n2:RIV%2F61388963%3A_____%2F09%3A00334373%21RIV10-MSM-61388963
rdf:type
skos:Concept n16:Vysledek
dcterms:description
A set of base-modified 2'-deoxyadenosine derivatives was incorporated to diverse DNA sequences by primer extension and the influence of the modification on cleavage by diverse restriction endonucleases was studied. While 8-substituted adenine derivatives were well tolerated by the enzymes, the presence of 7-substituted 7-deazaadenine in the recognition sequence resulted in blocking of cleavage by some enzymes depending on the nature and size of the 7-substituent. The results are useful both for protection of some sequences from cleavage and for manipulation of functionalized DNA by restriction cleavage. A set of base-modified 2'-deoxyadenosine derivatives was incorporated to diverse DNA sequences by primer extension and the influence of the modification on cleavage by diverse restriction endonucleases was studied. While 8-substituted adenine derivatives were well tolerated by the enzymes, the presence of 7-substituted 7-deazaadenine in the recognition sequence resulted in blocking of cleavage by some enzymes depending on the nature and size of the 7-substituent. The results are useful both for protection of some sequences from cleavage and for manipulation of functionalized DNA by restriction cleavage.
dcterms:title
Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases
skos:prefLabel
Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases
skos:notation
RIV/61388963:_____/09:00334373!RIV10-MSM-61388963
n3:aktivita
n7:Z n7:P
n3:aktivity
P(GA203/09/0317), P(LC512), Z(AV0Z40550506)
n3:cisloPeriodika
22
n3:dodaniDat
n17:2010
n3:domaciTvurceVysledku
n13:2810824 n13:6659160
n3:druhVysledku
n12:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
307256
n3:idVysledku
RIV/61388963:_____/09:00334373
n3:jazykVysledku
n11:eng
n3:klicovaSlova
functionalized DNA; restriction endonucleases; DNA polymerase; modified adenosines
n3:klicoveSlovo
n15:functionalized%20DNA n15:DNA%20polymerase n15:modified%20adenosines n15:restriction%20endonucleases
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[5468F5B47D31]
n3:nazevZdroje
Nucleic Acids Research
n3:obor
n14:CC
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n10:GA203%2F09%2F0317 n10:LC512
n3:rokUplatneniVysledku
n17:2009
n3:svazekPeriodika
37
n3:tvurceVysledku
Hocek, Michal Macíčková-Cahová, Hana
n3:wos
000272935000032
n3:zamer
n6:AV0Z40550506
s:issn
0305-1048
s:numberOfPages
11