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Statements

Subject Item
n2:RIV%2F61388955%3A_____%2F14%3A00433922%21RIV15-GA0-61388955
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Time-dependent fluorescence shift (TDFS) of Laurdan embedded in phospholipid bilayers reports on hydration and mobility of the phospholipid acylgroups. Exchange of H2O with D2O prolongs the lifetime of lipid-water and lipid-water-lipid interactions, which is reflected in a significantly slower TDFS kinetics. Combining TDFS measurements in H2O and D2O hydrated bilayers with atomistic molecular dynamics (MD) simulations provides a unique tool for characterization of the hydrogen bonding at the acylgroup level of lipid bilayers. In this work, we use this approach to study the influence of fluoride anions on the properties of cationic bilayers composed of trimethylammonium-propane (DOTAP). The results obtained for DOTAP are confronted with those for neutral phosphatidylcholine (DOPC) bilayers. Both in DOTAP and DOPC H2O/D2O exchange prolongs hydrogen-bonding lifetime and does not disturb bilayer structure. These results are confirmed by MD simulations. TDFS experiments show, however, that for DOTAP this effect is cancelled in the presence of fluoride ions. We interpret these results as evidence that strongly hydrated fluoride is able to steal water molecules that bridge lipid carbonyls. Consequently, when attracted to DOTAP bilayer, fluoride disrupts the local hydrogen-bonding network, and the differences in TDFS kinetics between H2O and D2O hydrated bilayers are no longer observed. A distinct behavior of fluoride is also evidenced by MD simulations, which show different lipid-ion binding for Cl and F. Time-dependent fluorescence shift (TDFS) of Laurdan embedded in phospholipid bilayers reports on hydration and mobility of the phospholipid acylgroups. Exchange of H2O with D2O prolongs the lifetime of lipid-water and lipid-water-lipid interactions, which is reflected in a significantly slower TDFS kinetics. Combining TDFS measurements in H2O and D2O hydrated bilayers with atomistic molecular dynamics (MD) simulations provides a unique tool for characterization of the hydrogen bonding at the acylgroup level of lipid bilayers. In this work, we use this approach to study the influence of fluoride anions on the properties of cationic bilayers composed of trimethylammonium-propane (DOTAP). The results obtained for DOTAP are confronted with those for neutral phosphatidylcholine (DOPC) bilayers. Both in DOTAP and DOPC H2O/D2O exchange prolongs hydrogen-bonding lifetime and does not disturb bilayer structure. These results are confirmed by MD simulations. TDFS experiments show, however, that for DOTAP this effect is cancelled in the presence of fluoride ions. We interpret these results as evidence that strongly hydrated fluoride is able to steal water molecules that bridge lipid carbonyls. Consequently, when attracted to DOTAP bilayer, fluoride disrupts the local hydrogen-bonding network, and the differences in TDFS kinetics between H2O and D2O hydrated bilayers are no longer observed. A distinct behavior of fluoride is also evidenced by MD simulations, which show different lipid-ion binding for Cl and F.
dcterms:title
Does fluoride disrupt hydrogen bond network in cationic lipid bilayer? Time-dependent fluorescence shift of Laurdan and molecular dynamics simulations Does fluoride disrupt hydrogen bond network in cationic lipid bilayer? Time-dependent fluorescence shift of Laurdan and molecular dynamics simulations
skos:prefLabel
Does fluoride disrupt hydrogen bond network in cationic lipid bilayer? Time-dependent fluorescence shift of Laurdan and molecular dynamics simulations Does fluoride disrupt hydrogen bond network in cationic lipid bilayer? Time-dependent fluorescence shift of Laurdan and molecular dynamics simulations
skos:notation
RIV/61388955:_____/14:00433922!RIV15-GA0-61388955
n3:aktivita
n4:P n4:I
n3:aktivity
I, P(GBP208/12/G016)
n3:cisloPeriodika
22
n3:dodaniDat
n6:2015
n3:domaciTvurceVysledku
n13:7569467 n13:6619789 n13:3982181
n3:druhVysledku
n17:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
12091
n3:idVysledku
RIV/61388955:_____/14:00433922
n3:jazykVysledku
n7:eng
n3:klicovaSlova
fluorescence sfifts; Cationic lipids; Hydrogen bond networks
n3:klicoveSlovo
n5:Cationic%20lipids n5:Hydrogen%20bond%20networks n5:fluorescence%20sfifts
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[E015AF8C8E9B]
n3:nazevZdroje
Journal of Chemical Physics
n3:obor
n9:CF
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
6
n3:projekt
n12:GBP208%2F12%2FG016
n3:rokUplatneniVysledku
n6:2014
n3:svazekPeriodika
141
n3:tvurceVysledku
Vazdar, M. Cwiklik, Lukasz Hof, Martin Jungwirth, Pavel Jurkiewicz, Piotr Pokorná, Šárka
n3:wos
000346272800063
s:issn
0021-9606
s:numberOfPages
9
n11:doi
10.1063/1.4898798