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Statements

Subject Item
n2:RIV%2F61388955%3A_____%2F14%3A00428759%21RIV15-GA0-61388955
rdf:type
n4:Vysledek skos:Concept
dcterms:description
Enzymatic reactor based on the powder of silver solid amalgam was suggested as the main part of biosensors in flow systems for the first time. 4-aminothiophenol, glutaraldehyde and enzyme were gradually bonded to the amalgam surface. Large surface of the fine amalgam particles maintains a big quantity of enzyme molecules. Biosensors were tested with enzymes ascorbate oxidase, glucose oxidase, catalase, tyrosinase and laccase. Electrochemical detection of the oxygen concentration change (for the first 3 enzymes) or the quinones concentration (tyrosinase and laccase) in the measured solution was done amperometrically by means of the tubular detector of silver solid amalgam. The current response of each biosensor was optimized with respect to the detection potential, the flow rate of the mobile phase, the injection volume and the enzymatic reactor volume. Under the found optimum conditions, concentration dependence and some statistical parameters of repeated measurements (relative standard deviation (RSD) for the studied enzymes was in the range 0.81 – 2.1 %) were measured. Biosensor with the ascorbate oxidase reactor was used for determination of ascorbic acid in the vitamin tablet Celaskon. Results of the analysis were in good agreement with the contents of ascorbic acid declared by manufacturer and the RSD of these analyses was 2.0 %. Enzymatic reactor based on the powder of silver solid amalgam was suggested as the main part of biosensors in flow systems for the first time. 4-aminothiophenol, glutaraldehyde and enzyme were gradually bonded to the amalgam surface. Large surface of the fine amalgam particles maintains a big quantity of enzyme molecules. Biosensors were tested with enzymes ascorbate oxidase, glucose oxidase, catalase, tyrosinase and laccase. Electrochemical detection of the oxygen concentration change (for the first 3 enzymes) or the quinones concentration (tyrosinase and laccase) in the measured solution was done amperometrically by means of the tubular detector of silver solid amalgam. The current response of each biosensor was optimized with respect to the detection potential, the flow rate of the mobile phase, the injection volume and the enzymatic reactor volume. Under the found optimum conditions, concentration dependence and some statistical parameters of repeated measurements (relative standard deviation (RSD) for the studied enzymes was in the range 0.81 – 2.1 %) were measured. Biosensor with the ascorbate oxidase reactor was used for determination of ascorbic acid in the vitamin tablet Celaskon. Results of the analysis were in good agreement with the contents of ascorbic acid declared by manufacturer and the RSD of these analyses was 2.0 %.
dcterms:title
Electrochemical Biosensors Based on Enzymatic Reactor with Amalgam Powder Electrochemical Biosensors Based on Enzymatic Reactor with Amalgam Powder
skos:prefLabel
Electrochemical Biosensors Based on Enzymatic Reactor with Amalgam Powder Electrochemical Biosensors Based on Enzymatic Reactor with Amalgam Powder
skos:notation
RIV/61388955:_____/14:00428759!RIV15-GA0-61388955
n3:aktivita
n7:I n7:P
n3:aktivity
I, P(GAP206/11/1638), P(GAP208/12/1645)
n3:dodaniDat
n14:2015
n3:domaciTvurceVysledku
n9:4407334 n9:1839594
n3:druhVysledku
n11:D
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
14099
n3:idVysledku
RIV/61388955:_____/14:00428759
n3:jazykVysledku
n10:eng
n3:klicovaSlova
Electrochemical biosensors; Flow analysis; Amperometry
n3:klicoveSlovo
n12:Electrochemical%20biosensors n12:Flow%20analysis n12:Amperometry
n3:kontrolniKodProRIV
[6BDB2DE08F2F]
n3:mistoKonaniAkce
Jetřichovice
n3:mistoVydani
Ústí nad Labem
n3:nazevZdroje
Sborník přednášek mezinárodní odborné konference XXXIV. Moderní Elektrochemické Metody
n3:obor
n13:CG
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n8:GAP206%2F11%2F1638 n8:GAP208%2F12%2F1645
n3:rokUplatneniVysledku
n14:2014
n3:tvurceVysledku
Josypčuk, Bohdan Barek, J. Josypčuk, Oksana
n3:typAkce
n20:EUR
n3:zahajeniAkce
2014-05-19+02:00
s:numberOfPages
5
n6:hasPublisher
Best Servis
n17:isbn
978-80-905221-2-1