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Statements

Subject Item
n2:RIV%2F61388955%3A_____%2F13%3A00392185%21RIV14-GA0-61388955
rdf:type
skos:Concept n16:Vysledek
dcterms:description
A flow amperometric enzymatic biosensor for the determination of glucose was constructed. The biosensor consists of a flow reactor based on porous silver solid amalgam (AgSA) and a flow tubular detector based on compact AgSA. The preparation of the sensor and the determination of glucose occurred in three steps. First, a self-assembled monolayer of 11-mercaptoundecanoic acid (MUA) was formed at the porous surface of the reactor. Second, enzyme glucose oxidase (GOx) was covalently immobilized at MUA-layer using N-ethyl-N-(3-dimethylaminopropyl) carboimide and N-hydroxysuccinimide chemistry. Finally, a decrease of oxygen concentration (directly proportional to the concentration of glucose) during enzymatic reaction was amperometrically measured on the tubular detector under flow injection conditions. The following parameters of glucose determination were optimized with respect to amperometric response: composition of the mobile phase, its concentration, the potential of detection and the flow rate. The calibration curve of glucose was linear in the concentration range of 0.02–0.80 mmol L1 with detection limit of 0.01 mmol L1. The content of glucose in the sample of honey was determined as 35.5 1.0 mass % (number of the repeated measurements n = 7; standard deviation SD = 1.2%; relative standard deviation RSD = 3.2%) which corresponds well with the declared values. The tested biosensor proved good long-term stability (77% of the current response of glucose was retained after 35 days). A flow amperometric enzymatic biosensor for the determination of glucose was constructed. The biosensor consists of a flow reactor based on porous silver solid amalgam (AgSA) and a flow tubular detector based on compact AgSA. The preparation of the sensor and the determination of glucose occurred in three steps. First, a self-assembled monolayer of 11-mercaptoundecanoic acid (MUA) was formed at the porous surface of the reactor. Second, enzyme glucose oxidase (GOx) was covalently immobilized at MUA-layer using N-ethyl-N-(3-dimethylaminopropyl) carboimide and N-hydroxysuccinimide chemistry. Finally, a decrease of oxygen concentration (directly proportional to the concentration of glucose) during enzymatic reaction was amperometrically measured on the tubular detector under flow injection conditions. The following parameters of glucose determination were optimized with respect to amperometric response: composition of the mobile phase, its concentration, the potential of detection and the flow rate. The calibration curve of glucose was linear in the concentration range of 0.02–0.80 mmol L1 with detection limit of 0.01 mmol L1. The content of glucose in the sample of honey was determined as 35.5 1.0 mass % (number of the repeated measurements n = 7; standard deviation SD = 1.2%; relative standard deviation RSD = 3.2%) which corresponds well with the declared values. The tested biosensor proved good long-term stability (77% of the current response of glucose was retained after 35 days).
dcterms:title
Flow electrochemical biosensors based on enzymatic porous reactor and tubular detector of silver solid amalgam Flow electrochemical biosensors based on enzymatic porous reactor and tubular detector of silver solid amalgam
skos:prefLabel
Flow electrochemical biosensors based on enzymatic porous reactor and tubular detector of silver solid amalgam Flow electrochemical biosensors based on enzymatic porous reactor and tubular detector of silver solid amalgam
skos:notation
RIV/61388955:_____/13:00392185!RIV14-GA0-61388955
n16:predkladatel
n19:ico%3A61388955
n3:aktivita
n8:P n8:I
n3:aktivity
I, P(GAP206/11/1638)
n3:cisloPeriodika
MAY 2013
n3:dodaniDat
n17:2014
n3:domaciTvurceVysledku
n18:1839594 n18:4407334
n3:druhVysledku
n9:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
75315
n3:idVysledku
RIV/61388955:_____/13:00392185
n3:jazykVysledku
n5:eng
n3:klicovaSlova
flow analysis; amperometry; silver solid amalgam
n3:klicoveSlovo
n10:flow%20analysis n10:silver%20solid%20amalgam n10:amperometry
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[086B45FCC835]
n3:nazevZdroje
Analytica Chimica Acta
n3:obor
n14:CF
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n7:GAP206%2F11%2F1638
n3:rokUplatneniVysledku
n17:2013
n3:svazekPeriodika
778
n3:tvurceVysledku
Josypčuk, Oksana Barek, J. Josypčuk, Bohdan
n3:wos
000318959400004
s:issn
0003-2670
s:numberOfPages
7
n12:doi
10.1016/j.aca.2013.03.055