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Statements

Subject Item
n2:RIV%2F61388955%3A_____%2F12%3A00384160%21RIV13-GA0-61388955
rdf:type
skos:Concept n6:Vysledek
dcterms:description
Hydrogen cyanide (HCN) and 2-aminoacetophenone (2-AA; H2NC6H4COCH3) are possible biomarkers of pulmonary Pseudomonas aeruginosa (PA) infection that could be used in an exhaled breath test. All factors affecting their production need to be investigated, including the culture conditions: planktonic (free-floating) or biofilm (non-motile communities attached to a solid surface). In vivo, the change from planktonic to biofilm growth is signalled when a certain population density is reached. Using selected ion flow tube mass spectrometry, SIFT-MS, we have analysed HCN and 2-AA produced by 12 genotyped PA samples, cultured under both planktonic and biofilm conditions after 24, 48, 72 and 96 hours of incubation. The 12 samples included 3 different strains (genotypes), 50% of which had a mucoid phenotype and 50% had a non-mucoid phenotype. All samples produced significant concentrations of HCN; median (25th to 75th percentiles, IQR) concentration: 144 (61-512) parts-per-billion by volume (ppbv). Multivariate analysis showed HCN production varied dependent on genotype (p = 0.0014), culture duration (p = 0.005) and phenotype (p < 0.001) but not culture conditions (planktonic/biofilm). Much smaller concentrations of 2-AA were detected, median (IQR) concentration 1.8 (1.3-3) ppbv, despite which, multivariate analysis showed production was affected by genotype (p < 0.001) and culture duration (p = 0.007) but not phenotype or culture conditions. These data show that biofilm formation does not affect HCN production by PA and supports its use as a biomarker of PA infection. The concentrations of 2-AA are much lower than previous studies have shown. The reason for this is unclear but it raises questions about its suitability as a biomarker of PA infection. Hydrogen cyanide (HCN) and 2-aminoacetophenone (2-AA; H2NC6H4COCH3) are possible biomarkers of pulmonary Pseudomonas aeruginosa (PA) infection that could be used in an exhaled breath test. All factors affecting their production need to be investigated, including the culture conditions: planktonic (free-floating) or biofilm (non-motile communities attached to a solid surface). In vivo, the change from planktonic to biofilm growth is signalled when a certain population density is reached. Using selected ion flow tube mass spectrometry, SIFT-MS, we have analysed HCN and 2-AA produced by 12 genotyped PA samples, cultured under both planktonic and biofilm conditions after 24, 48, 72 and 96 hours of incubation. The 12 samples included 3 different strains (genotypes), 50% of which had a mucoid phenotype and 50% had a non-mucoid phenotype. All samples produced significant concentrations of HCN; median (25th to 75th percentiles, IQR) concentration: 144 (61-512) parts-per-billion by volume (ppbv). Multivariate analysis showed HCN production varied dependent on genotype (p = 0.0014), culture duration (p = 0.005) and phenotype (p < 0.001) but not culture conditions (planktonic/biofilm). Much smaller concentrations of 2-AA were detected, median (IQR) concentration 1.8 (1.3-3) ppbv, despite which, multivariate analysis showed production was affected by genotype (p < 0.001) and culture duration (p = 0.007) but not phenotype or culture conditions. These data show that biofilm formation does not affect HCN production by PA and supports its use as a biomarker of PA infection. The concentrations of 2-AA are much lower than previous studies have shown. The reason for this is unclear but it raises questions about its suitability as a biomarker of PA infection.
dcterms:title
Quantification of hydrogen cyanide and 2-aminoacetophenone in the headspace of Pseudomonas aeruginosa cultured under biofilm and planktonic conditions Quantification of hydrogen cyanide and 2-aminoacetophenone in the headspace of Pseudomonas aeruginosa cultured under biofilm and planktonic conditions
skos:prefLabel
Quantification of hydrogen cyanide and 2-aminoacetophenone in the headspace of Pseudomonas aeruginosa cultured under biofilm and planktonic conditions Quantification of hydrogen cyanide and 2-aminoacetophenone in the headspace of Pseudomonas aeruginosa cultured under biofilm and planktonic conditions
skos:notation
RIV/61388955:_____/12:00384160!RIV13-GA0-61388955
n6:predkladatel
n7:ico%3A61388955
n3:aktivita
n16:P n16:I
n3:aktivity
I, P(GA203/09/0256)
n3:cisloPeriodika
11
n3:dodaniDat
n10:2013
n3:domaciTvurceVysledku
n4:9912479
n3:druhVysledku
n14:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
163788
n3:idVysledku
RIV/61388955:_____/12:00384160
n3:jazykVysledku
n19:eng
n3:klicovaSlova
tube mass spectrometry; cystic fibrosis; volatile metabolites
n3:klicoveSlovo
n5:tube%20mass%20spectrometry n5:cystic%20fibrosis n5:volatile%20metabolites
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[5D6059B39CCC]
n3:nazevZdroje
Analytical Methods: advancing methods and applications
n3:obor
n18:CF
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
9
n3:projekt
n9:GA203%2F09%2F0256
n3:rokUplatneniVysledku
n10:2012
n3:svazekPeriodika
4
n3:tvurceVysledku
Lenney, W. Smith, D. Jones, A. M. Sims, H. Španěl, Patrik Gilchrist, F. J. Belcher, J. Alcock, A. Webb, A. K.
n3:wos
000310281700025
s:issn
1759-9660
s:numberOfPages
5
n13:doi
10.1039/c2ay25652e