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Statements

Subject Item
n2:RIV%2F61388955%3A_____%2F10%3A00342982%21RIV11-MSM-61388955
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Lipopolythioureas (LPT) are original non cationic systems representing an alternative to cationic lipids. Their high transfection efficiency prompted us to investigate further their biophysical properties, and in particular how thiourea lipids interact with DNA. The interaction of lipopolythiourea with DNA was investigated by fluorescence correlation microscopy (FCS). Influence of the lipid length and nature of the thiourea head on the thiourea/DNA interaction were studied. FCS revealed a strong interaction between lipopolythiourea and DNA, occurring at 1 equivalent of a thiourea lipid by a DNA phosphate group, and leading to a condensed plasmid state. From previous in vitro experiments, we could conclude that the lipid leading to the more condensed state of DNA was also the more efficient to transfect cells. Lipopolythioureas (LPT) are original non cationic systems representing an alternative to cationic lipids. Their high transfection efficiency prompted us to investigate further their biophysical properties, and in particular how thiourea lipids interact with DNA. The interaction of lipopolythiourea with DNA was investigated by fluorescence correlation microscopy (FCS). Influence of the lipid length and nature of the thiourea head on the thiourea/DNA interaction were studied. FCS revealed a strong interaction between lipopolythiourea and DNA, occurring at 1 equivalent of a thiourea lipid by a DNA phosphate group, and leading to a condensed plasmid state. From previous in vitro experiments, we could conclude that the lipid leading to the more condensed state of DNA was also the more efficient to transfect cells.
dcterms:title
Lipopolythiourea/DNA interaction: A biophysical study Lipopolythiourea/DNA interaction: A biophysical study
skos:prefLabel
Lipopolythiourea/DNA interaction: A biophysical study Lipopolythiourea/DNA interaction: A biophysical study
skos:notation
RIV/61388955:_____/10:00342982!RIV11-MSM-61388955
n3:aktivita
n4:P n4:Z
n3:aktivity
P(IAA400400621), P(LC06063), Z(AV0Z40400503)
n3:cisloPeriodika
1-3
n3:dodaniDat
n9:2011
n3:domaciTvurceVysledku
Kral, Teresa n12:3982181
n3:druhVysledku
n8:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
268611
n3:idVysledku
RIV/61388955:_____/10:00342982
n3:jazykVysledku
n16:eng
n3:klicovaSlova
lipopolythiourea; FCS; plasmid DNA compaction; 31P NMR
n3:klicoveSlovo
n5:FCS n5:31P%20NMR n5:plasmid%20DNA%20compaction n5:lipopolythiourea
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[BF0D39CA4A01]
n3:nazevZdroje
Biophysical Chemistry
n3:obor
n17:CF
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
6
n3:projekt
n14:IAA400400621 n14:LC06063
n3:rokUplatneniVysledku
n9:2010
n3:svazekPeriodika
148
n3:tvurceVysledku
Scherman, D. Leblond, J. Mignet, N. Hof, Martin Kral, Teresa Hersovici, J.
n3:wos
000277743900009
n3:zamer
n15:AV0Z40400503
s:issn
0301-4622
s:numberOfPages
6