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Statements

Subject Item
n2:RIV%2F61388955%3A_____%2F07%3A00089208%21RIV08-MSM-61388955
rdf:type
n14:Vysledek skos:Concept
dcterms:description
Fluorescenční antibunching je známá metoda pro určování počtu nezávislých emiterů přítomných v molekulárních komplexech. Tato metoda byla ale zřídka aplikována pro studium autofluorescenčních proteinů imobilizovaných na povrchu kvůli jejich nízké fotostabilitě. V této práci jsme měřili fluorescenční antibunching v roztoku a nasbírali tak data z obrovského počtu molekul, což nám umožnilo určit stechiometrii komplexů těchto fotolabilních proteinů. Tato metoda navíc nevyžaduje kalibraci standardem. Fluorescence antibunching is a well-known technique for determining the number of independent emitters per molecule or molecular complex. It was rarely applied to autofluorescent proteins due to the necessity of collecting large numbers of fluorescence photons from a single molecule, which is usually impossible to achieve with rather photolabile autofluorescent proteins. Here, we measure fluorescence antibunching on molecules in solution, allowing us to accumulate data over a large number of molecules. We use that method for determining an average stoichiometry of molecular complexes. The proposed method is absolute in the sense that it does not need any calibration or referencing. We develop the necessary theoretical background and check the method on pure dye solutions and on molecular complexes with known stoichiometry. Fluorescence antibunching is a well-known technique for determining the number of independent emitters per molecule or molecular complex. It was rarely applied to autofluorescent proteins due to the necessity of collecting large numbers of fluorescence photons from a single molecule, which is usually impossible to achieve with rather photolabile autofluorescent proteins. Here, we measure fluorescence antibunching on molecules in solution, allowing us to accumulate data over a large number of molecules. We use that method for determining an average stoichiometry of molecular complexes. The proposed method is absolute in the sense that it does not need any calibration or referencing. We develop the necessary theoretical background and check the method on pure dye solutions and on molecular complexes with known stoichiometry.
dcterms:title
Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes Měření “antibunchingu“ fluorescence v roztoku: cesta k určení stechiometrie molekulovýxch komplexů
skos:prefLabel
Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes Měření “antibunchingu“ fluorescence v roztoku: cesta k určení stechiometrie molekulovýxch komplexů Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes
skos:notation
RIV/61388955:_____/07:00089208!RIV08-MSM-61388955
n3:strany
4040;4049
n3:aktivita
n12:P n12:Z
n3:aktivity
P(LC06063), Z(AV0Z40400503)
n3:cisloPeriodika
-
n3:dodaniDat
n17:2008
n3:domaciTvurceVysledku
n5:7173784
n3:druhVysledku
n15:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
421348
n3:idVysledku
RIV/61388955:_____/07:00089208
n3:jazykVysledku
n8:eng
n3:klicovaSlova
exploring fluorescence antibunching; molecular complexes; biophysical methods
n3:klicoveSlovo
n9:biophysical%20methods n9:molecular%20complexes n9:exploring%20fluorescence%20antibunching
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[4E3C98155462]
n3:nazevZdroje
Analytical Chemistry
n3:obor
n18:CF
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:projekt
n10:LC06063
n3:rokUplatneniVysledku
n17:2007
n3:svazekPeriodika
79
n3:tvurceVysledku
Sýkora, Jan Bönigk, W. Enderlain, J. Gregor, I. Schmalzing, G. Kaiser, K.
n3:zamer
n11:AV0Z40400503
s:issn
0003-2700
s:numberOfPages
10