This HTML5 document contains 45 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n18http://linked.opendata.cz/resource/domain/vavai/projekt/
n16http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n14http://linked.opendata.cz/ontology/domain/vavai/
n12http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n17http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F61388955%3A_____%2F04%3A00101099%21RIV%2F2005%2FAV0%2FA54005%2FN/
n5http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n11http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n15http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n13http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n10http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n8http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F61388955%3A_____%2F04%3A00101099%21RIV%2F2005%2FAV0%2FA54005%2FN
rdf:type
skos:Concept n14:Vysledek
dcterms:description
A simple amperometric detector with a platinum tubular electrode was tested for the determination of urinary 8-hydroxy-2''-deoxyguanosine (8-OHdG) by high-performance liquid chromatography. Analyte was extracted from human urine using a single-step solid-phase extraction with the average recovery of 71 +/- 7%. Analysis was carried out on a C-18 analytical column under isocratic conditions. The limit of detection of 8-OHdG of 3 nM (S/N = 3) was comparable with that achieved using the conventional amperometric detector. Procedure was used for the determination of 8-OHdG levels in 10 obese patients (body-mass index > 30 kg m(-2)) yielding the mean concentration 32 +/- 27.1 nM of 8-OHdG or 1.85 +/- 0.72 .mu.mol 8-OHdG mol(-1) creatinine (4.63 +/- 1.8. mu.g 8-OHdG g(-1) creatinine), which is not much different from the levels found in healthy individuals. Pro analýzu 8-hydroxy-2´-deoxyguanosinu (8-OHdG) v moči obézních pacientů metodou HPLC byl testován jednoduchý amperometrický detektor. Analyt byl extrahován z moči s použitím jednostupňové extrakce na pevné fázi s průměrným výtěžkem 71%. HPLC byla prováděna na C-18 analytických kolonách za izokratických podmínek. Zjištěný limit detekce 8-OHdG (3nM) byl srovnatelný s limitem dosaženým při použití komerčně dostupného amperometrického detektoru. Postup měření byl pak použit pro určení hladin 8-OHdG v moči 10 obézních pacientů s výsledkem 32+/-27,1 nM, který se příliš nelišil od hodnoty zjištěné u zdravých jedinců. A simple amperometric detector with a platinum tubular electrode was tested for the determination of urinary 8-hydroxy-2''-deoxyguanosine (8-OHdG) by high-performance liquid chromatography. Analyte was extracted from human urine using a single-step solid-phase extraction with the average recovery of 71 +/- 7%. Analysis was carried out on a C-18 analytical column under isocratic conditions. The limit of detection of 8-OHdG of 3 nM (S/N = 3) was comparable with that achieved using the conventional amperometric detector. Procedure was used for the determination of 8-OHdG levels in 10 obese patients (body-mass index > 30 kg m(-2)) yielding the mean concentration 32 +/- 27.1 nM of 8-OHdG or 1.85 +/- 0.72 .mu.mol 8-OHdG mol(-1) creatinine (4.63 +/- 1.8. mu.g 8-OHdG g(-1) creatinine), which is not much different from the levels found in healthy individuals.
dcterms:title
Analýza 8-hydroxy-2´-deoxyguanosinu v moči obézních pacientů metodou HPLC s elektrochemickou detekcí Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection
skos:prefLabel
Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection Analýza 8-hydroxy-2´-deoxyguanosinu v moči obézních pacientů metodou HPLC s elektrochemickou detekcí Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection
skos:notation
RIV/61388955:_____/04:00101099!RIV/2005/AV0/A54005/N
n4:strany
107;110
n4:aktivita
n9:Z n9:P
n4:aktivity
P(GA203/01/0653), Z(AV0Z4040901)
n4:cisloPeriodika
-
n4:dodaniDat
n8:2005
n4:domaciTvurceVysledku
n16:5904196
n4:druhVysledku
n13:J
n4:duvernostUdaju
n11:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
560141
n4:idVysledku
RIV/61388955:_____/04:00101099
n4:jazykVysledku
n15:eng
n4:klicovaSlova
8-hydroxy-2''''-deoxyguanosine; DNA; obese patient
n4:klicoveSlovo
n5:obese%20patient n5:DNA n5:8-hydroxy-2%27%27%27%27-deoxyguanosine
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[FC0E0D1541D4]
n4:nazevZdroje
Analytica Chimica Acta
n4:obor
n10:CF
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
4
n4:projekt
n18:GA203%2F01%2F0653
n4:rokUplatneniVysledku
n8:2004
n4:svazekPeriodika
516
n4:tvurceVysledku
Opekar, F. Samcová, E. Langmaier, Jan Marhol, P.
n4:zamer
n12:AV0Z4040901
s:issn
0003-2670
s:numberOfPages
4