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Statements

Subject Item
n2:RIV%2F61388955%3A_____%2F03%3A54030048%21RIV%2F2004%2FAV0%2FA54004%2FN
rdf:type
skos:Concept n12:Vysledek
dcterms:description
Confocal fluorescence correlation spectroscopy (FCS) allows for the determination of lateral diffusion coefficients and surface densities in planar phospholipid systems. The determination of the z-position of the laser focus relative to the phospholipid surface plane is of crucial importance for the accuracy of the confocal FCS experiment. In this work we are determining for the first time this z-position of the laser focus by a so-called 'z-scan', which is based on the determination of diffusion times and particle numbers in 0.1 m steps along the z-axis. Experiments on supported phospholipid bilayers composed of dioleoyl-phosphatidylcholine (DOPC) and small amounts of Rhodamine Red-X 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Rhodamine Red-X DHPE) adsorbed onto atomically flat mica demonstrate that results obtained by the 'z-scan' approach are significantly more precise than those results obtained when the fluorescence. Confocal fluorescence correlation spectroscopy (FCS) allows for the determination of lateral diffusion coefficients and surface densities in planar phospholipid systems. The determination of the z-position of the laser focus relative to the phospholipid surface plane is of crucial importance for the accuracy of the confocal FCS experiment. In this work we are determining for the first time this z-position of the laser focus by a so-called 'z-scan', which is based on the determination of diffusion times and particle numbers in 0.1 m steps along the z-axis. Experiments on supported phospholipid bilayers composed of dioleoyl-phosphatidylcholine (DOPC) and small amounts of Rhodamine Red-X 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Rhodamine Red-X DHPE) adsorbed onto atomically flat mica demonstrate that results obtained by the 'z-scan' approach are significantly more precise than those results obtained when the fluorescence.
dcterms:title
How to Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy. How to Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy.
skos:prefLabel
How to Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy. How to Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy.
skos:notation
RIV/61388955:_____/03:54030048!RIV/2004/AV0/A54004/N
n3:strany
4120;4126
n3:aktivita
n11:P n11:Z
n3:aktivity
P(LN00A032), Z(AV0Z4040901)
n3:cisloPeriodika
N/A
n3:dodaniDat
n15:2004
n3:domaciTvurceVysledku
n9:6933254 n9:6774199 n9:3728536 n9:3982181 n9:2455439
n3:druhVysledku
n5:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
609366
n3:idVysledku
RIV/61388955:_____/03:54030048
n3:jazykVysledku
n13:eng
n3:klicovaSlova
fluorescence; diffusion; spectroscopy
n3:klicoveSlovo
n10:fluorescence n10:diffusion n10:spectroscopy
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[F0388443F53B]
n3:nazevZdroje
Langmuir
n3:obor
n8:CF
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n14:LN00A032
n3:rokUplatneniVysledku
n15:2003
n3:svazekPeriodika
19
n3:tvurceVysledku
Hermens, W. Th. Benda, Aleš Mareček, Vladimír Hof, Martin Beneš, Martin Lhotský, Alexandr
n3:zamer
n4:AV0Z4040901
s:issn
0743-7463
s:numberOfPages
7