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Statements

Subject Item
n2:RIV%2F60646594%3A_____%2F11%3A%230000011%21RIV14-MPO-60646594
rdf:type
skos:Concept n13:Vysledek
dcterms:description
Raman spectroscopy is a powerful tool for chemical analysis. This technique can elucidate fundamental questions about the metabolic processes and intercellular variability on a single cell level. Therefore, Raman spectroscopy can significantly contribute to the study and use of microalgae in systems biology and biofuel technology. Raman spectroscopy can be combined with optical tweezers. We have employed microfluidic system to deliver the sampled microalgae to the Raman-tweezers. This instrument is able to measure chemical composition of cells and to track metabolic processes in vivo, in real-time and label-free making it possible to detect population variability in a wide array of traits. Moreover, employing an active sorting switch, cells can be separated depending on input parameters obtained from Raman spectra. We focus on algal lipids which are promising potential products for biofuel as well as for nutrition. Important parameter characterizing the algal lipids is the degree of unsaturation of the constituent fatty acids. We demonstrate the capacity of our Raman tweezers based sensor to sort cells according to the degree of unsaturation in lipid storage bodies of individual living algal cells. Raman spectroscopy is a powerful tool for chemical analysis. This technique can elucidate fundamental questions about the metabolic processes and intercellular variability on a single cell level. Therefore, Raman spectroscopy can significantly contribute to the study and use of microalgae in systems biology and biofuel technology. Raman spectroscopy can be combined with optical tweezers. We have employed microfluidic system to deliver the sampled microalgae to the Raman-tweezers. This instrument is able to measure chemical composition of cells and to track metabolic processes in vivo, in real-time and label-free making it possible to detect population variability in a wide array of traits. Moreover, employing an active sorting switch, cells can be separated depending on input parameters obtained from Raman spectra. We focus on algal lipids which are promising potential products for biofuel as well as for nutrition. Important parameter characterizing the algal lipids is the degree of unsaturation of the constituent fatty acids. We demonstrate the capacity of our Raman tweezers based sensor to sort cells according to the degree of unsaturation in lipid storage bodies of individual living algal cells.
dcterms:title
Raman microspectroscopy based sensor of algal lipid unsaturation Raman microspectroscopy based sensor of algal lipid unsaturation
skos:prefLabel
Raman microspectroscopy based sensor of algal lipid unsaturation Raman microspectroscopy based sensor of algal lipid unsaturation
skos:notation
RIV/60646594:_____/11:#0000011!RIV14-MPO-60646594
n13:predkladatel
n14:ico%3A60646594
n3:aktivita
n5:P n5:Z
n3:aktivity
P(ED0017/01/01), P(FR-TI1/433), P(OC08034), Z(AV0Z20650511)
n3:dodaniDat
n16:2014
n3:domaciTvurceVysledku
n22:2172704
n3:druhVysledku
n23:D
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n21:predkladatel
n3:idSjednocenehoVysledku
225645
n3:idVysledku
RIV/60646594:_____/11:#0000011
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Raman spectroscopy; algal cells; lipids; iodine value
n3:klicoveSlovo
n7:lipids n7:iodine%20value n7:algal%20cells n7:Raman%20spectroscopy
n3:kontrolniKodProRIV
[57E1E51B5F08]
n3:mistoKonaniAkce
Praha
n3:mistoVydani
Bellingham
n3:nazevZdroje
Optical Sensors 2011 and Photonic Crystal Fibers V (Proceedings of SPIE Vol. 8073)
n3:obor
n6:BH
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
9
n3:projekt
n18:OC08034 n18:FR-TI1%2F433 n18:ED0017%2F01%2F01
n3:rokUplatneniVysledku
n16:2011
n3:tvurceVysledku
Šerý, M. Jonáš, A. Samek, O. Nedbal, L. Bernatová, S. Pilát, Z. Ježek, J. Zemánek, P. Trtílek, Martin
n3:typAkce
n20:WRD
n3:wos
000293740400014
n3:zahajeniAkce
2011-04-18+02:00
n3:zamer
n8:AV0Z20650511
s:numberOfPages
6
n4:doi
10.1117/12.886750
n15:hasPublisher
SPIE
n17:isbn
978-0-8194-8663-9