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Statements

Subject Item
n2:RIV%2F60461373%3A22340%2F13%3A43895115%21RIV14-GA0-22340___
rdf:type
n5:Vysledek skos:Concept
dcterms:description
The locations of three bilirubin (BR)-binding sites with different affinities were identified as subdomains IB, IIA and IIIA for five mammalian serum albumins (SAs): human (HSA), bovine (BSA), rat, (RSA), rabbit (RbSA) and sheep (SSA). The stereoselectivity of a high-affinity BR-binding site was identified in the BR/SA=1/1 system by circular dichroism (CD) spectroscopy, the sites with low affinity to BR were analyzed using difference CD. Site-specific ligand-competition experiments with ibuprofen (marker for subdomain IIIA) and hemin (marker for subdomain IB) did not reveal any changes for the BR/SA=1/1 system and showed a decrease of the bound BR at BR/SA=3/1. Both sites were identified as sites with low affinity to BR. The correlation between stereoselectivity and the arrangement of Arg-Lys residues indicated similarity between the BR-binding sites in subdomain IIIA for all of the SAs studied. Subdomain IB in HSA, BSA, SSA and RbSA has P-stereoselectivity while in RSA it has M-selectivity toward BR. A ligand-competition experiment with gossypol shows a decrease of the CD signal of bound BR for the BR/SA=1/1 system as well as for BR/SA=3/1. Subdomain IIA was assigned as a high-affinity BR-binding site. The P-stereoselectivity of this site in HSA (and RSA, RbSA) was caused by the right-hand localization of charged residues R257/R218-R222, whereas the left-hand orientation of R257/R218-R199 led to the M-stereoselectivity of the primary binding site in BSA (and SSA). The locations of three bilirubin (BR)-binding sites with different affinities were identified as subdomains IB, IIA and IIIA for five mammalian serum albumins (SAs): human (HSA), bovine (BSA), rat, (RSA), rabbit (RbSA) and sheep (SSA). The stereoselectivity of a high-affinity BR-binding site was identified in the BR/SA=1/1 system by circular dichroism (CD) spectroscopy, the sites with low affinity to BR were analyzed using difference CD. Site-specific ligand-competition experiments with ibuprofen (marker for subdomain IIIA) and hemin (marker for subdomain IB) did not reveal any changes for the BR/SA=1/1 system and showed a decrease of the bound BR at BR/SA=3/1. Both sites were identified as sites with low affinity to BR. The correlation between stereoselectivity and the arrangement of Arg-Lys residues indicated similarity between the BR-binding sites in subdomain IIIA for all of the SAs studied. Subdomain IB in HSA, BSA, SSA and RbSA has P-stereoselectivity while in RSA it has M-selectivity toward BR. A ligand-competition experiment with gossypol shows a decrease of the CD signal of bound BR for the BR/SA=1/1 system as well as for BR/SA=3/1. Subdomain IIA was assigned as a high-affinity BR-binding site. The P-stereoselectivity of this site in HSA (and RSA, RbSA) was caused by the right-hand localization of charged residues R257/R218-R222, whereas the left-hand orientation of R257/R218-R199 led to the M-stereoselectivity of the primary binding site in BSA (and SSA).
dcterms:title
The location of the high- and low-affinity bilirubin-binding sites on serum albumin: Ligand-competition analysis investigated by circular dichroism The location of the high- and low-affinity bilirubin-binding sites on serum albumin: Ligand-competition analysis investigated by circular dichroism
skos:prefLabel
The location of the high- and low-affinity bilirubin-binding sites on serum albumin: Ligand-competition analysis investigated by circular dichroism The location of the high- and low-affinity bilirubin-binding sites on serum albumin: Ligand-competition analysis investigated by circular dichroism
skos:notation
RIV/60461373:22340/13:43895115!RIV14-GA0-22340___
n5:predkladatel
n14:orjk%3A22340
n3:aktivita
n15:S n15:P
n3:aktivity
P(GAP206/11/0836), S
n3:cisloPeriodika
22 June 2013
n3:dodaniDat
n11:2014
n3:domaciTvurceVysledku
n7:6564844 n7:1422251 n7:6364136
n3:druhVysledku
n8:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
85280
n3:idVysledku
RIV/60461373:22340/13:43895115
n3:jazykVysledku
n9:eng
n3:klicovaSlova
gossypol; difference circular dichroism; dCD; circular dichroism; bovine serum albumin; bilirubin; Stereoselectivity; SSA; SA; RbSA; RSA; PSB; Low affinity; Ligand-completion; Ibf; High affinity; HSA; GS; CD; Binding site; Bilirubin; BSA; BR
n3:klicoveSlovo
n4:RbSA n4:bilirubin n4:SA n4:RSA n4:dCD n4:CD n4:GS n4:PSB n4:gossypol n4:Binding%20site n4:difference%20circular%20dichroism n4:BSA n4:circular%20dichroism n4:Ibf n4:Low%20affinity n4:HSA n4:Stereoselectivity n4:Bilirubin n4:bovine%20serum%20albumin n4:SSA n4:High%20affinity n4:BR n4:Ligand-completion
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[B0AE939DAF7F]
n3:nazevZdroje
Biophysical Chemistry
n3:obor
n19:CB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n6:GAP206%2F11%2F0836
n3:rokUplatneniVysledku
n11:2013
n3:svazekPeriodika
180-181
n3:tvurceVysledku
Goncharová, Iryna Urbanová, Marie Orlov, Sergey
n3:wos
000324898700007
s:issn
1873-4200
s:numberOfPages
11
n20:doi
10.1016/j.bpc.2013.06.004
n10:organizacniJednotka
22340