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Statements

Subject Item
n2:RIV%2F60461373%3A22340%2F13%3A43895054%21RIV14-MZ0-22340___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Chiroptical methods are widely used in structural and conformational analyses of biopolymers. The application of these methods to investigations of biofluids would provide new avenues for the molecular diagnosis of protein-misfolding diseases. In this work, samples of human blood plasma and hen egg white were analyzed using a combination of conventional and chiroptical methods: ultraviolet absorption/electronic circular dichroism (UV/ECD), Fourier transform infrared absorption/vibrational circular dichroism (FTIR/VCD), and Raman scattering/Raman optical activity (Raman/ROA). For comparison, the main components of these substances-human serum albumin (HSA) and ovalbumin (Ova)-were also analyzed by these methods. The ultraviolet region of the ECD spectrum was analyzed using the CDNN CD software package to evaluate the secondary structures of the proteins. The UV/ECD, FTIR/VCD, and Raman/ROA spectra of the substances were quite similar to those of the corresponding major proteins, while some differences were also detected and explained. The conclusions drawn from the FTIR/VCD and Raman/ROA data were in good agreement with the secondary structures calculated from ECD. The results obtained in this work demonstrate that the chiroptical methods used here can be applied to analyze not only pure protein solutions but also more complex systems, such as biological fluids. Chiroptical methods are widely used in structural and conformational analyses of biopolymers. The application of these methods to investigations of biofluids would provide new avenues for the molecular diagnosis of protein-misfolding diseases. In this work, samples of human blood plasma and hen egg white were analyzed using a combination of conventional and chiroptical methods: ultraviolet absorption/electronic circular dichroism (UV/ECD), Fourier transform infrared absorption/vibrational circular dichroism (FTIR/VCD), and Raman scattering/Raman optical activity (Raman/ROA). For comparison, the main components of these substances-human serum albumin (HSA) and ovalbumin (Ova)-were also analyzed by these methods. The ultraviolet region of the ECD spectrum was analyzed using the CDNN CD software package to evaluate the secondary structures of the proteins. The UV/ECD, FTIR/VCD, and Raman/ROA spectra of the substances were quite similar to those of the corresponding major proteins, while some differences were also detected and explained. The conclusions drawn from the FTIR/VCD and Raman/ROA data were in good agreement with the secondary structures calculated from ECD. The results obtained in this work demonstrate that the chiroptical methods used here can be applied to analyze not only pure protein solutions but also more complex systems, such as biological fluids.
dcterms:title
Analysis of human blood plasma and hen egg white by chiroptical spectroscopic methods (ECD, VCD, ROA) Analysis of human blood plasma and hen egg white by chiroptical spectroscopic methods (ECD, VCD, ROA)
skos:prefLabel
Analysis of human blood plasma and hen egg white by chiroptical spectroscopic methods (ECD, VCD, ROA) Analysis of human blood plasma and hen egg white by chiroptical spectroscopic methods (ECD, VCD, ROA)
skos:notation
RIV/60461373:22340/13:43895054!RIV14-MZ0-22340___
n16:predkladatel
n17:orjk%3A22340
n3:aktivita
n11:Z n11:I n11:S n11:P
n3:aktivity
I, P(GAP208/11/0105), P(NT13259), S, Z(MSM6046137307)
n3:cisloPeriodika
16
n3:dodaniDat
n13:2014
n3:domaciTvurceVysledku
n7:2744112 n7:7696310 n7:9594752 n7:3002675 n7:8250065
n3:druhVysledku
n19:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
60557
n3:idVysledku
RIV/60461373:22340/13:43895054
n3:jazykVysledku
n5:eng
n3:klicovaSlova
Chiroptical spectroscopy; Ovalbumin; Human serum albumin; Hen egg white; Human blood plasma
n3:klicoveSlovo
n10:Ovalbumin n10:Hen%20egg%20white n10:Human%20serum%20albumin n10:Chiroptical%20spectroscopy n10:Human%20blood%20plasma
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[49DD3C40DFD5]
n3:nazevZdroje
Analytical and Bioanalytical Chemistry
n3:obor
n15:CB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
7
n3:projekt
n12:NT13259 n12:GAP208%2F11%2F0105
n3:rokUplatneniVysledku
n13:2013
n3:svazekPeriodika
405
n3:tvurceVysledku
Judexová, Miluše Hrubý, Tomáš Synytsya, Alla Tatarkovič, Michal Petruželka, Luboš Miškovicová, Michaela Setnička, Vladimír
n3:wos
000319745700009
n3:zamer
n18:MSM6046137307
s:issn
1618-2642
s:numberOfPages
13
n20:doi
10.1007/s00216-013-6946-6
n21:organizacniJednotka
22340