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Statements

Subject Item
n2:RIV%2F60461373%3A22340%2F12%3A43893654%21RIV13-MSM-22340___
rdf:type
n18:Vysledek skos:Concept
dcterms:description
We hereby present the development of an electrochemical biosensor utilizing enzyme immunoassay for detection of various protein molecules. The aim is to fabricate a versatile cost available biosensor with at least same or lower detection limits as commonly used techniques. The detection of a model analyte relies on the monitoring redox reaction which is catalyzed by an enzyme marker. The analyte is marked with the horseradish peroxidase (HRP) enzyme. The electrochemical methods for the purpose of detection via the monitoring of redox reaction were cyclic voltammetry and electrochemical impedance spectroscopy (EIS). A microfluidic chip with incorporated gold electrodes was fabricated for this purpose. Here we present measurements with HRP enzyme without immobilization and development of immobilization of proteins on gold electrodes studied by electrochemical impedance spectroscopy. We hereby present the development of an electrochemical biosensor utilizing enzyme immunoassay for detection of various protein molecules. The aim is to fabricate a versatile cost available biosensor with at least same or lower detection limits as commonly used techniques. The detection of a model analyte relies on the monitoring redox reaction which is catalyzed by an enzyme marker. The analyte is marked with the horseradish peroxidase (HRP) enzyme. The electrochemical methods for the purpose of detection via the monitoring of redox reaction were cyclic voltammetry and electrochemical impedance spectroscopy (EIS). A microfluidic chip with incorporated gold electrodes was fabricated for this purpose. Here we present measurements with HRP enzyme without immobilization and development of immobilization of proteins on gold electrodes studied by electrochemical impedance spectroscopy.
dcterms:title
Study on electrochemical biosensors for detection of bioaffinity complexes Study on electrochemical biosensors for detection of bioaffinity complexes
skos:prefLabel
Study on electrochemical biosensors for detection of bioaffinity complexes Study on electrochemical biosensors for detection of bioaffinity complexes
skos:notation
RIV/60461373:22340/12:43893654!RIV13-MSM-22340___
n18:predkladatel
n22:orjk%3A22340
n3:aktivita
n4:P n4:S
n3:aktivity
P(IAA401280904), S
n3:dodaniDat
n8:2013
n3:domaciTvurceVysledku
n13:1795880 n13:6965032 n13:8915407
n3:druhVysledku
n20:D
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
172245
n3:idVysledku
RIV/60461373:22340/12:43893654
n3:jazykVysledku
n17:eng
n3:klicovaSlova
electrochemical impedance spectroscopy; horseradish peroxidase; biosensor; microfluidic device
n3:klicoveSlovo
n6:horseradish%20peroxidase n6:microfluidic%20device n6:biosensor n6:electrochemical%20impedance%20spectroscopy
n3:kontrolniKodProRIV
[1ECEB98D2FBC]
n3:mistoKonaniAkce
Tatranské Matliare
n3:mistoVydani
Bratislava
n3:nazevZdroje
39th International Conference of Slovak Society of Chemical Engineering
n3:obor
n10:CI
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n12:IAA401280904
n3:rokUplatneniVysledku
n8:2012
n3:tvurceVysledku
Přibyl, Michal Fišer, Jan Červenka, Petr
n3:typAkce
n9:WRD
n3:zahajeniAkce
2012-05-21+02:00
s:numberOfPages
8
n19:hasPublisher
Slovak Society of Chemical Engineering
n21:isbn
978-80-89475-04-9
n16:organizacniJednotka
22340