This HTML5 document contains 48 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n18http://localhost/temp/predkladatel/
n17http://linked.opendata.cz/resource/domain/vavai/projekt/
n5http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n15http://linked.opendata.cz/resource/domain/vavai/subjekt/
n7http://linked.opendata.cz/ontology/domain/vavai/
n8http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n13http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n4http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n21http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n20http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n16http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n10http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n6http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F60461373%3A22340%2F12%3A43893316%21RIV13-GA0-22340___/
n19http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F60461373%3A22340%2F12%3A43893316%21RIV13-GA0-22340___
rdf:type
n7:Vysledek skos:Concept
dcterms:description
In this study we investigated the possibility of using infrared spectroscopy for the characterization of the ligand (diethylenetriaminepentaacetic acid, DTPA) in various aqueous media (water and different buffers), the antibody (immunoglobulin G, IgG), the metal-ligand complexes (Y-DTPA and Lu-DTPA) and the metal-ligand-antibody conjugates (Y-DTPA-IgG and Lu-DTPA-IgG). The aim was to describe behaviour of individual components, and to find spectral markers of complexes and/or conjugates formation. The reason of the choice of Lu and Y was the potential use of their radionuclides to form radiopharmaceutical species for nuclear medicine. We performed kinetic measurements to describe the stability/instability of individual systems. Multivariate data analysis allowed finding out spectral regions related to different chemical processes. Hydrolysis effect of the diethylenetriaminepentaacetic acid dianhydride (cDTPAA) in water was confirmed in unbuffered aqueous solution. Nevertheless, the study of cDTPAA in various buffered media showed evident suppression of hydrolysis. Thus, appropriate buffers were selected as media for complexation reactions with Y and Lu. Characteristic marker bands of complexation were identified. Human IgG in phosphate buffer and its interaction with the metal-ligand complexes were studied. Observation of new bands, band shifts and intensity changes revealed formation of the metal-ligand-IgG conjugates together with a somewhat changed secondary structure of IgG. We demonstrated the possibility of vibrational spectroscopic monitoring of the preparation steps of the metal-ligand-antibody conjugates as potential radiopharmaceutical products. In this study we investigated the possibility of using infrared spectroscopy for the characterization of the ligand (diethylenetriaminepentaacetic acid, DTPA) in various aqueous media (water and different buffers), the antibody (immunoglobulin G, IgG), the metal-ligand complexes (Y-DTPA and Lu-DTPA) and the metal-ligand-antibody conjugates (Y-DTPA-IgG and Lu-DTPA-IgG). The aim was to describe behaviour of individual components, and to find spectral markers of complexes and/or conjugates formation. The reason of the choice of Lu and Y was the potential use of their radionuclides to form radiopharmaceutical species for nuclear medicine. We performed kinetic measurements to describe the stability/instability of individual systems. Multivariate data analysis allowed finding out spectral regions related to different chemical processes. Hydrolysis effect of the diethylenetriaminepentaacetic acid dianhydride (cDTPAA) in water was confirmed in unbuffered aqueous solution. Nevertheless, the study of cDTPAA in various buffered media showed evident suppression of hydrolysis. Thus, appropriate buffers were selected as media for complexation reactions with Y and Lu. Characteristic marker bands of complexation were identified. Human IgG in phosphate buffer and its interaction with the metal-ligand complexes were studied. Observation of new bands, band shifts and intensity changes revealed formation of the metal-ligand-IgG conjugates together with a somewhat changed secondary structure of IgG. We demonstrated the possibility of vibrational spectroscopic monitoring of the preparation steps of the metal-ligand-antibody conjugates as potential radiopharmaceutical products.
dcterms:title
Infrared spectroscopic study of the model metal-ligand-antibody systems: What information on the structure and stability of systems can be obtained? Infrared spectroscopic study of the model metal-ligand-antibody systems: What information on the structure and stability of systems can be obtained?
skos:prefLabel
Infrared spectroscopic study of the model metal-ligand-antibody systems: What information on the structure and stability of systems can be obtained? Infrared spectroscopic study of the model metal-ligand-antibody systems: What information on the structure and stability of systems can be obtained?
skos:notation
RIV/60461373:22340/12:43893316!RIV13-GA0-22340___
n7:predkladatel
n15:orjk%3A22340
n3:aktivita
n20:P n20:Z
n3:aktivity
P(GAP206/11/0951), Z(MSM6046137307)
n3:cisloPeriodika
1
n3:dodaniDat
n19:2013
n3:domaciTvurceVysledku
n5:1954415 n5:7290659 n5:1280422
n3:druhVysledku
n14:J
n3:duvernostUdaju
n21:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
141832
n3:idVysledku
RIV/60461373:22340/12:43893316
n3:jazykVysledku
n16:eng
n3:klicovaSlova
Partial least squares regression; Immunoconjugate; FTIR spectroscopy; Lutetium; Yttrium
n3:klicoveSlovo
n4:Immunoconjugate n4:FTIR%20spectroscopy n4:Lutetium n4:Yttrium n4:Partial%20least%20squares%20regression
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[5EEE2124A413]
n3:nazevZdroje
Vibrational Spectroscopy
n3:obor
n10:CB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n17:GAP206%2F11%2F0951
n3:rokUplatneniVysledku
n19:2012
n3:svazekPeriodika
61
n3:tvurceVysledku
Křížová, Věra Reková, Marie Matějka, Pavel
n3:wos
000304686400012
n3:zamer
n8:MSM6046137307
s:issn
0924-2031
s:numberOfPages
7
n13:doi
10.1016/j.vibspec.2012.02.011
n18:organizacniJednotka
22340