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Statements

Subject Item
n2:RIV%2F60461373%3A22340%2F09%3A00021878%21RIV10-MSM-22340___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Fractionation of species of Cd, Cu, Mo, Ni and Zn in extracts of rye seedlings (grown up in both normal and Cd2+-enriched medium) and rye flour were performed by SEC/ICPMS method. Majority of Cu, Zn and Ni in all samples is bound in a 1-2 kDa fraction. Mo in all samples occurs in a fraction of Mr of 3 kDa. During five days of cultivation in a solution of 30 mol l-1 Cd2+ the plants accumulated as much as 5 mg kg-1 of Cd, but its soluble portion represents only 12-15%. Prevailing portion of Cd complexes is involved in a fraction of 3 kDa, while the minor part occurs in a fraction of 20 kDa. The speciation of elevated Cd in plants differs from that of other metals present at a physiological level. The metal-rich fractions of extracts of all samples (i.e. those of Mr of 1-2 kDa) were refined by immobilised metal affinity chromatography. Isolated ligands of trace elements are peptides rich in dicarboxylic amino-acids. Fractionation of species of Cd, Cu, Mo, Ni and Zn in extracts of rye seedlings (grown up in both normal and Cd2+-enriched medium) and rye flour were performed by SEC/ICPMS method. Majority of Cu, Zn and Ni in all samples is bound in a 1-2 kDa fraction. Mo in all samples occurs in a fraction of Mr of 3 kDa. During five days of cultivation in a solution of 30 mol l-1 Cd2+ the plants accumulated as much as 5 mg kg-1 of Cd, but its soluble portion represents only 12-15%. Prevailing portion of Cd complexes is involved in a fraction of 3 kDa, while the minor part occurs in a fraction of 20 kDa. The speciation of elevated Cd in plants differs from that of other metals present at a physiological level. The metal-rich fractions of extracts of all samples (i.e. those of Mr of 1-2 kDa) were refined by immobilised metal affinity chromatography. Isolated ligands of trace elements are peptides rich in dicarboxylic amino-acids.
dcterms:title
Trace elements species fractionation in rye flour and rye (Secale cereale) seedlings Trace elements species fractionation in rye flour and rye (Secale cereale) seedlings
skos:prefLabel
Trace elements species fractionation in rye flour and rye (Secale cereale) seedlings Trace elements species fractionation in rye flour and rye (Secale cereale) seedlings
skos:notation
RIV/60461373:22340/09:00021878!RIV10-MSM-22340___
n3:aktivita
n15:Z
n3:aktivity
Z(MSM6046137305), Z(MSM6046137307)
n3:cisloPeriodika
1
n3:dodaniDat
n9:2010
n3:domaciTvurceVysledku
n4:9046976 n4:1120530 n4:8440646 n4:1250558 n4:2901153
n3:druhVysledku
n8:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
346612
n3:idVysledku
RIV/60461373:22340/09:00021878
n3:jazykVysledku
n18:eng
n3:klicovaSlova
rye; speciation; trace elements; MALDI-MS; ICP-MS; IMAC
n3:klicoveSlovo
n12:ICP-MS n12:speciation n12:rye n12:IMAC n12:MALDI-MS n12:trace%20elements
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[DA44F51589FB]
n3:nazevZdroje
Czech Journal of Food Science
n3:obor
n10:CB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n9:2009
n3:svazekPeriodika
27
n3:tvurceVysledku
Šantrůček, Jiří Komínková, Jana Polák, Jan Mestek, Oto Koplík, Richard Kodíček, Milan
n3:wos
000264402600005
n3:zamer
n11:MSM6046137307 n11:MSM6046137305
s:issn
1212-1800
s:numberOfPages
11
n5:organizacniJednotka
22340