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Statements

Subject Item
n2:RIV%2F60461373%3A22340%2F06%3A00016651%21RIV07-MSM-22340___
rdf:type
n19:Vysledek skos:Concept
dcterms:description
Tato práce je zaměřena na vývoj experimentálního čipu pro heterogenní imunoanalýzu. Polystyren byl použit jako substrát pro výrobu čipu. Při výrobě byly použity techniky mikrofrézování a slinování za tepla. Byly studovány dvě metody detekce biologických molekul - lidských IgG: (i) jednokroková ProteinA-lidský IgG-FITC a (ii) sendvičová ProteinA - lidský IgG - kozí IgG-FITC. Byla vyvinuta imobiizační procedura a oběma technikami byly estrojeny kalibrační křivky závislosti fluorescenčního signálu na koncentraci IgG ve vzorku. This work is focused on development of an experimental microchip for heterogeneous immunoassay. Polystyrene substrate is used as the material for fabrication of the microfluidic chip. Techniques of hot embossing and micro-milling are employed in the fabrication. The central part of the chip contains immobilized protein molecules with biological affinity (Protein A - PA). Two procedures of PA immobilization have been studied: (i) passive sorption and (ii) covalent crosslinking of protein layers. It has been found that both these methods are proper. In the one-step assay, liquid samples containing the complementary molecules (human immunoglobulins FITC labeled - hIgG) are dosed in the place of the immobilized layer through the inlet/outlet microfluidic channels. A sandwich (multi-step) system Protein A - human immunoglobulins - goat immunoglobulins (FITC labeled - gIgG) is also studied. After washing out, the fluorescence intensity in microchannels is proportional to the amount of the formed This work is focused on development of an experimental microchip for heterogeneous immunoassay. Polystyrene substrate is used as the material for fabrication of the microfluidic chip. Techniques of hot embossing and micro-milling are employed in the fabrication. The central part of the chip contains immobilized protein molecules with biological affinity (Protein A - PA). Two procedures of PA immobilization have been studied: (i) passive sorption and (ii) covalent crosslinking of protein layers. It has been found that both these methods are proper. In the one-step assay, liquid samples containing the complementary molecules (human immunoglobulins FITC labeled - hIgG) are dosed in the place of the immobilized layer through the inlet/outlet microfluidic channels. A sandwich (multi-step) system Protein A - human immunoglobulins - goat immunoglobulins (FITC labeled - gIgG) is also studied. After washing out, the fluorescence intensity in microchannels is proportional to the amount of the formed
dcterms:title
Development of a microfluidic chip for heterogeneous immunoassay Vývoj mikrofluidního čipu pro heterogenní imunoanalýzu Development of a microfluidic chip for heterogeneous immunoassay
skos:prefLabel
Development of a microfluidic chip for heterogeneous immunoassay Vývoj mikrofluidního čipu pro heterogenní imunoanalýzu Development of a microfluidic chip for heterogeneous immunoassay
skos:notation
RIV/60461373:22340/06:00016651!RIV07-MSM-22340___
n5:strany
J1.4(1-7)
n5:aktivita
n7:Z n7:P
n5:aktivity
P(GP104/03/D006), Z(MSM6046137306)
n5:dodaniDat
n10:2007
n5:domaciTvurceVysledku
n12:6965032 n12:3133672
n5:druhVysledku
n16:D
n5:duvernostUdaju
n20:S
n5:entitaPredkladatele
n11:predkladatel
n5:idSjednocenehoVysledku
471268
n5:idVysledku
RIV/60461373:22340/06:00016651
n5:jazykVysledku
n9:eng
n5:klicovaSlova
heterogeneous immunoassay; microchip fabrication; antibody; Protein A; onestep assay; sandwich assay
n5:klicoveSlovo
n6:microchip%20fabrication n6:antibody n6:Protein%20A n6:sandwich%20assay n6:heterogeneous%20immunoassay n6:onestep%20assay
n5:kontrolniKodProRIV
[DF875AF1A9FA]
n5:mistoKonaniAkce
Praha
n5:mistoVydani
Praha
n5:nazevZdroje
Sborník 17th International Congress of Chemical and Process Engineering
n5:obor
n13:CI
n5:pocetDomacichTvurcuVysledku
2
n5:pocetTvurcuVysledku
5
n5:projekt
n22:GP104%2F03%2FD006
n5:rokUplatneniVysledku
n10:2006
n5:tvurceVysledku
Šnita, Dalimil Schrott, Walter Přibyl, Michal Pecháčková, Lucie Štěpánek, Jakub
n5:typAkce
n18:WRD
n5:zahajeniAkce
2006-08-27+02:00
n5:zamer
n17:MSM6046137306
s:numberOfPages
7
n4:hasPublisher
Czech Society of Chemical Engineering
n14:isbn
80-86059-45-6
n15:organizacniJednotka
22340