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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F14%3A43898424%21RIV15-MZE-22330___
rdf:type
n6:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0167701214003042
dcterms:description
Staphylococcus aureus produces a wide variety of superantigenic activity Staphylococcal enterotoxins (SE) and they are a major cause of food poisoning. These superantigens are associated with mobile genetic elements such as plasmids, prophages and S. aureus pathogenicity islands (SaPI). The presence of well-known eight SaPI integrase and 13 enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sel, sek, seq, and tst) in 93 S. aureus strains were investigated. All S. aureus isolates were characterized by pulsed-field gel electrophoresis (PFGE), and the genes were detected using five sets of multiplex PCR (mPCR). The most predominant toxin genes were sea (19%), seb (15%), sec (54%), sell (48%), selk (46%), selq (52%), seg (22%), and sei (19%). Analysis showed that many S. aureus isolates harbored multiple toxin genes. An mPCR-based assay was developed for the determination of all SaPI and their superantigen gene combinations. Twenty three isolates revealed the gene combination sec, sell and tst, typical of the SaPIbov1 and SaPIn1/m1 pathogenicity islands. Twelve isolates revealed the selk and selq gene combination consistent with SaPI3. Eight isolates exhibited the sec and sell genes without the tst gene typical of SaPImw2. We established a correlation between superantigenic toxin genotypes in S. aureus in terms of combinations of toxin gene-encoding SaPI. These results provide a rapid method for determining superantigenic toxin genotypes in S. aureus strains. A total of 24 PFGE patterns were generated. To our knowledge, this is a first study analyzing the correlation of all known SaPI and their enterotoxins in S. aureus using mPCR. Staphylococcus aureus produces a wide variety of superantigenic activity Staphylococcal enterotoxins (SE) and they are a major cause of food poisoning. These superantigens are associated with mobile genetic elements such as plasmids, prophages and S. aureus pathogenicity islands (SaPI). The presence of well-known eight SaPI integrase and 13 enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sel, sek, seq, and tst) in 93 S. aureus strains were investigated. All S. aureus isolates were characterized by pulsed-field gel electrophoresis (PFGE), and the genes were detected using five sets of multiplex PCR (mPCR). The most predominant toxin genes were sea (19%), seb (15%), sec (54%), sell (48%), selk (46%), selq (52%), seg (22%), and sei (19%). Analysis showed that many S. aureus isolates harbored multiple toxin genes. An mPCR-based assay was developed for the determination of all SaPI and their superantigen gene combinations. Twenty three isolates revealed the gene combination sec, sell and tst, typical of the SaPIbov1 and SaPIn1/m1 pathogenicity islands. Twelve isolates revealed the selk and selq gene combination consistent with SaPI3. Eight isolates exhibited the sec and sell genes without the tst gene typical of SaPImw2. We established a correlation between superantigenic toxin genotypes in S. aureus in terms of combinations of toxin gene-encoding SaPI. These results provide a rapid method for determining superantigenic toxin genotypes in S. aureus strains. A total of 24 PFGE patterns were generated. To our knowledge, this is a first study analyzing the correlation of all known SaPI and their enterotoxins in S. aureus using mPCR.
dcterms:title
Molecular analysis of Staphylococcus aureus pathogenicity islands (SaPI) and their superantigens combination of food samples Molecular analysis of Staphylococcus aureus pathogenicity islands (SaPI) and their superantigens combination of food samples
skos:prefLabel
Molecular analysis of Staphylococcus aureus pathogenicity islands (SaPI) and their superantigens combination of food samples Molecular analysis of Staphylococcus aureus pathogenicity islands (SaPI) and their superantigens combination of food samples
skos:notation
RIV/60461373:22330/14:43898424!RIV15-MZE-22330___
n3:aktivita
n19:S n19:P
n3:aktivity
P(QJ1210300), S
n3:cisloPeriodika
DEC 2014
n3:dodaniDat
n9:2015
n3:domaciTvurceVysledku
n10:3804879 n10:2493403 n10:6034926 n10:2857219
n3:druhVysledku
n20:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
30262
n3:idVysledku
RIV/60461373:22330/14:43898424
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Staphylococcus aureus, Enterotoxin gene, SaPI, PFGE, Food, mPCR
n3:klicoveSlovo
n4:Food n4:SaPI n4:Staphylococcus%20aureus n4:PFGE n4:mPCR n4:Enterotoxin%20gene
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[3FC1A6A2FB4F]
n3:nazevZdroje
Journal of Microbiological Methods
n3:obor
n15:EE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n13:QJ1210300
n3:rokUplatneniVysledku
n9:2014
n3:svazekPeriodika
107
n3:tvurceVysledku
Zdeňková, Kamila Sýkorová, Hana Demnerová, Kateřina Alibayov, Babek
n3:wos
000347605900033
s:issn
0167-7012
s:numberOfPages
8
n16:doi
10.1016/j.mimet.2014.10.014
n18:organizacniJednotka
22330