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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F14%3A43898073%21RIV15-MZE-22330___
rdf:type
n10:Vysledek skos:Concept
rdfs:seeAlso
http://onlinelibrary.wiley.com/doi/10.1002/jssc.201301292/abstract;jsessionid=11AA20E7EA8C3AF7ED627F3408DD5047.f04t02
dcterms:description
The ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UHPLC-QTOFMS) was applied to evaluate the potential of non-target metabolomic fingerprinting to distinguish Fusarium-infected and control barley samples. First, the sample extraction and UHPLC-QTOFMS instrumental conditions were optimized to obtain the broadest possible representation of polar/medium-polar compounds occurring in extracts obtained from barley grain samples. Next, the UHPLC-QTOFMS metabolomic fingerprints of extracts obtained from nine barley varieties were acquired under the conditions of electrospray ionization (ESI) in both positive and negative mode. Each variety of barley was tested in two variants: artificially infected by Fusarium culmorum at the beginning of heading and as control group (no infection). In addition, the dynamics of barley infection development was monitored using this approach. The experimental data were statistically evaluated by principal component analysis, hierarchical clustering analysis, and partial least squares discriminant analysis. Differentiation of barley in response to F. culmorum infection was feasible using this metabolomics-based method. Analysis in ESI(+) provided higher number of molecular features as compared to that performed under ESI(-) setting. However, the analysis in ESI(-) permitted the detection of deoxynivalenol and deoxynivalenol-3-glucoside considered as resistance-indicator metabolites in barley. The ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UHPLC-QTOFMS) was applied to evaluate the potential of non-target metabolomic fingerprinting to distinguish Fusarium-infected and control barley samples. First, the sample extraction and UHPLC-QTOFMS instrumental conditions were optimized to obtain the broadest possible representation of polar/medium-polar compounds occurring in extracts obtained from barley grain samples. Next, the UHPLC-QTOFMS metabolomic fingerprints of extracts obtained from nine barley varieties were acquired under the conditions of electrospray ionization (ESI) in both positive and negative mode. Each variety of barley was tested in two variants: artificially infected by Fusarium culmorum at the beginning of heading and as control group (no infection). In addition, the dynamics of barley infection development was monitored using this approach. The experimental data were statistically evaluated by principal component analysis, hierarchical clustering analysis, and partial least squares discriminant analysis. Differentiation of barley in response to F. culmorum infection was feasible using this metabolomics-based method. Analysis in ESI(+) provided higher number of molecular features as compared to that performed under ESI(-) setting. However, the analysis in ESI(-) permitted the detection of deoxynivalenol and deoxynivalenol-3-glucoside considered as resistance-indicator metabolites in barley.
dcterms:title
Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley
skos:prefLabel
Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley
skos:notation
RIV/60461373:22330/14:43898073!RIV15-MZE-22330___
n4:aktivita
n16:P
n4:aktivity
P(QI111B044)
n4:cisloPeriodika
8
n4:dodaniDat
n14:2015
n4:domaciTvurceVysledku
n5:7726481 n5:8580588 n5:3901556 n5:4423232 n5:7597037
n4:druhVysledku
n17:J
n4:duvernostUdaju
n12:S
n4:entitaPredkladatele
n6:predkladatel
n4:idSjednocenehoVysledku
41359
n4:idVysledku
RIV/60461373:22330/14:43898073
n4:jazykVysledku
n19:eng
n4:klicovaSlova
mycotoxins; metabolomics; liquid chromatography-mass spectrometry (LC-MS); Fusarium head blight; Barley
n4:klicoveSlovo
n7:metabolomics n7:Fusarium%20head%20blight n7:Barley n7:mycotoxins n7:liquid%20chromatography-mass%20spectrometry%20%28LC-MS%29
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[B1E3CE70D91B]
n4:nazevZdroje
Journal of Separation Science
n4:obor
n11:GH
n4:pocetDomacichTvurcuVysledku
5
n4:pocetTvurcuVysledku
6
n4:projekt
n9:QI111B044
n4:rokUplatneniVysledku
n14:2014
n4:svazekPeriodika
37
n4:tvurceVysledku
Ovesná, Jaroslava Džuman, Zbyněk Čajka, Tomáš Hajšlová, Jana Václavík, Lukáš Václavíková, Marta
n4:wos
000334059900003
s:issn
1615-9306
s:numberOfPages
8
n18:doi
10.1002/jssc.201301292
n13:organizacniJednotka
22330