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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F14%3A43898023%21RIV15-TA0-22330___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Several laboratories had tested bioactivity of the materials in commercially available solution DMEM (Dulbecco's Modified Eagle's Medium) that is normally used for cultivation of cell cultures. The objective of this work was to find out whether it is possible to replace TRIS-buffered SBF currently used for bioactivity tests with the non-buffered DMEM solution. To understand the role of the organic part of the DMEM solution in the process of crystallization, we have prepared non-buffered solution simulating only its inorganic part (identified as I-solution). It was found that under static-dynamic test conditions calcite (CaCO3) and the amorphous phase of calcium phosphate (ACP) formed on the surface of the glassceramic (45S5 bioactive glass based) scaffold exposed to both solutions. Additionally, halite (NaCl) formed at the beginning of exposure to DMEM. Hydroxyapatite phase was not detected on the surface in either non-buffered solution. Organic components contained in the DMEM solution failed to prevent formation of crystalline phases. The present results indicate that it is not recommendable to use DMEM for bioactivity tests of glass-ceramic materials due to its low concentration of Ca2+ ions, high concentration of HCO3 ions and the necessity to maintain sterile environment during the test. Several laboratories had tested bioactivity of the materials in commercially available solution DMEM (Dulbecco's Modified Eagle's Medium) that is normally used for cultivation of cell cultures. The objective of this work was to find out whether it is possible to replace TRIS-buffered SBF currently used for bioactivity tests with the non-buffered DMEM solution. To understand the role of the organic part of the DMEM solution in the process of crystallization, we have prepared non-buffered solution simulating only its inorganic part (identified as I-solution). It was found that under static-dynamic test conditions calcite (CaCO3) and the amorphous phase of calcium phosphate (ACP) formed on the surface of the glassceramic (45S5 bioactive glass based) scaffold exposed to both solutions. Additionally, halite (NaCl) formed at the beginning of exposure to DMEM. Hydroxyapatite phase was not detected on the surface in either non-buffered solution. Organic components contained in the DMEM solution failed to prevent formation of crystalline phases. The present results indicate that it is not recommendable to use DMEM for bioactivity tests of glass-ceramic materials due to its low concentration of Ca2+ ions, high concentration of HCO3 ions and the necessity to maintain sterile environment during the test.
dcterms:title
Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests? Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?
skos:prefLabel
Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests? Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?
skos:notation
RIV/60461373:22330/14:43898023!RIV15-TA0-22330___
n4:aktivita
n13:P
n4:aktivity
P(TE01020390)
n4:cisloPeriodika
2014
n4:dodaniDat
n5:2015
n4:domaciTvurceVysledku
n8:3655326
n4:druhVysledku
n14:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n12:predkladatel
n4:idSjednocenehoVysledku
22901
n4:idVysledku
RIV/60461373:22330/14:43898023
n4:jazykVysledku
n11:eng
n4:klicovaSlova
static-dynamic conditions, buffer TRIS; in vitro test; DMEM; SBF; calcite; hydroxyapatite; glass-ceramics; scaffold; Bioactivity
n4:klicoveSlovo
n6:Bioactivity n6:scaffold n6:DMEM n6:glass-ceramics n6:hydroxyapatite n6:calcite n6:in%20vitro%20test n6:SBF n6:static-dynamic%20conditions n6:buffer%20TRIS
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[8D6F06A98769]
n4:nazevZdroje
Journal of Materials Chemistry
n4:obor
n15:CA
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
6
n4:projekt
n19:TE01020390
n4:rokUplatneniVysledku
n5:2014
n4:svazekPeriodika
2
n4:tvurceVysledku
Horkavcová, Diana Častorálová, Markéta Rohanová, Dana Bozděchová, Pavlína Bezdička, Petr Boccaccini, Aldo Roberto
n4:wos
000339861000013
s:issn
0959-9428
s:numberOfPages
9
n9:doi
10.1039/c4tb00187g
n16:organizacniJednotka
22330