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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F14%3A43897784%21RIV15-GA0-22330___
rdf:type
skos:Concept n15:Vysledek
rdfs:seeAlso
http://ac.els-cdn.com/S0167701214000207/1-s2.0-S0167701214000207-main.pdf?_tid=0fe3df92-a861-11e4-8f66-00000aab0f6c&acdnat=1422609736_0d9b8721de703da1123ae5ee6262e32d
dcterms:description
Intact cell MALDI-TOF mass spectrometry is a rapid tool for the identification and classification of microorganisms, now widely used even in clinical laboratories. However, its distinctive power is not sufficient for some closely-related species. The genus Cronobacter, formerly known as Enterobacter sakazakii, contains such species. In this work, a new method for the differentiation of five Cronobacter species is presented involving the tryptic digestion of cytoplasmatic proteins followed by MALDI mass spectrometry analysis. A database was developed for use in Bruker Biotyper software including 52 reference spectra and tested on a set of 45 samples with an overall accuracy of about 80%. The possibility of measurement automation and the short time and low cost requirements of this method compared to those of biochemical tests or PCR methods make it a supplementary option to intact cell MALDI, providing additional information about the differentiation of problematic species. Intact cell MALDI-TOF mass spectrometry is a rapid tool for the identification and classification of microorganisms, now widely used even in clinical laboratories. However, its distinctive power is not sufficient for some closely-related species. The genus Cronobacter, formerly known as Enterobacter sakazakii, contains such species. In this work, a new method for the differentiation of five Cronobacter species is presented involving the tryptic digestion of cytoplasmatic proteins followed by MALDI mass spectrometry analysis. A database was developed for use in Bruker Biotyper software including 52 reference spectra and tested on a set of 45 samples with an overall accuracy of about 80%. The possibility of measurement automation and the short time and low cost requirements of this method compared to those of biochemical tests or PCR methods make it a supplementary option to intact cell MALDI, providing additional information about the differentiation of problematic species.
dcterms:title
Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis
skos:prefLabel
Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis
skos:notation
RIV/60461373:22330/14:43897784!RIV15-GA0-22330___
n5:aktivita
n17:I n17:S n17:P
n5:aktivity
I, P(GAP503/10/0664), S
n5:cisloPeriodika
March
n5:dodaniDat
n14:2015
n5:domaciTvurceVysledku
n8:3623068 n8:6936903 n8:2565412 n8:8488681 n8:1120530
n5:druhVysledku
n18:J
n5:duvernostUdaju
n6:S
n5:entitaPredkladatele
n10:predkladatel
n5:idSjednocenehoVysledku
11500
n5:idVysledku
RIV/60461373:22330/14:43897784
n5:jazykVysledku
n13:eng
n5:klicovaSlova
Trypsin; Mass spectrometry; MALDI-TOF; Cronobacter; Biotyper
n5:klicoveSlovo
n7:Biotyper n7:Trypsin n7:Cronobacter n7:Mass%20spectrometry n7:MALDI-TOF
n5:kodStatuVydavatele
NL - Nizozemsko
n5:kontrolniKodProRIV
[584F7B2B94F2]
n5:nazevZdroje
Journal of Microbiological Methods
n5:obor
n19:CE
n5:pocetDomacichTvurcuVysledku
5
n5:pocetTvurcuVysledku
6
n5:projekt
n20:GAP503%2F10%2F0664
n5:rokUplatneniVysledku
n14:2014
n5:svazekPeriodika
98
n5:tvurceVysledku
Krásný, Lukáš Rohlová, Eva Růžičková, Helena Šantrůček, Jiří Hochel, Igor Hynek, Radovan
n5:wos
000333852700019
s:issn
0167-7012
s:numberOfPages
9
n11:doi
10.1016/j.mimet.2014.01.008
n9:organizacniJednotka
22330