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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F14%3A43896443%21RIV15-MSM-22330___
rdf:type
n5:Vysledek skos:Concept
dcterms:description
Simultaneous saccharification and fermentation (SSF) of lignocellulosic substrate is limited by low glucose availability in the early stages of SSF, different temperature optima for enzymatic hydrolysis and fermentation, and low ethanol productivity. These were eliminated in this work by carrying out the pre-saccharification step at the optimum temperature for cellulolytic enzymes. When inoculation was delayed for 12 h (dSSF process), ethanol productivity was enhanced and the process was shortened by 60% compared to SSF, while the final ethanol concentration remained the same. Increased loading of acid-pretreated wheat straw in batch mode did not improve the ethanol concentration; although preconcentration of substrate from 36% to 66% of wet solid enabled a 48% increase in glucose concentration it negatively influenced fermentation. The fed-batch dSSF process, with gradual feeding of pre-hydrolysed medium, carried out in two bioreactors, enabled us to increase the final ethanol concentration and to eliminate problems associated with high concentrations of inhibitors in batch mode and high viscosity of the medium. Simultaneous saccharification and fermentation (SSF) of lignocellulosic substrate is limited by low glucose availability in the early stages of SSF, different temperature optima for enzymatic hydrolysis and fermentation, and low ethanol productivity. These were eliminated in this work by carrying out the pre-saccharification step at the optimum temperature for cellulolytic enzymes. When inoculation was delayed for 12 h (dSSF process), ethanol productivity was enhanced and the process was shortened by 60% compared to SSF, while the final ethanol concentration remained the same. Increased loading of acid-pretreated wheat straw in batch mode did not improve the ethanol concentration; although preconcentration of substrate from 36% to 66% of wet solid enabled a 48% increase in glucose concentration it negatively influenced fermentation. The fed-batch dSSF process, with gradual feeding of pre-hydrolysed medium, carried out in two bioreactors, enabled us to increase the final ethanol concentration and to eliminate problems associated with high concentrations of inhibitors in batch mode and high viscosity of the medium.
dcterms:title
High solid fed-batch SSF with delayed inoculation for improved production of bioethanol from wheat straw High solid fed-batch SSF with delayed inoculation for improved production of bioethanol from wheat straw
skos:prefLabel
High solid fed-batch SSF with delayed inoculation for improved production of bioethanol from wheat straw High solid fed-batch SSF with delayed inoculation for improved production of bioethanol from wheat straw
skos:notation
RIV/60461373:22330/14:43896443!RIV15-MSM-22330___
n3:aktivita
n19:P
n3:aktivity
P(ME10146)
n3:cisloPeriodika
únor
n3:dodaniDat
n9:2015
n3:domaciTvurceVysledku
n8:4521137 n8:3943305 n8:2569868 n8:6816517
n3:druhVysledku
n4:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
19095
n3:idVysledku
RIV/60461373:22330/14:43896443
n3:jazykVysledku
n16:eng
n3:klicovaSlova
high solid; fed-batch; saccharification; wheat straw; Bioethanol
n3:klicoveSlovo
n7:wheat%20straw n7:saccharification n7:Bioethanol n7:high%20solid n7:fed-batch
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[6ED5FF37CF53]
n3:nazevZdroje
Fuel
n3:obor
n6:EI
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n13:ME10146
n3:rokUplatneniVysledku
n9:2014
n3:svazekPeriodika
122
n3:tvurceVysledku
Paulová, Leona Melzoch, Karel Rychtera, Mojmír Patáková, Petra
n3:wos
000331544100034
s:issn
0016-2361
s:numberOfPages
7
n18:doi
10.1016/j.fuel.2014.01.020
n12:organizacniJednotka
22330