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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F14%3A43895925%21RIV15-MZE-22330___
rdf:type
skos:Concept n17:Vysledek
rdfs:seeAlso
http://link.springer.com/article/10.1007/s00284-013-0512-5
dcterms:description
We propose a new method that allows accurate discrimination of Lactobacillus helveticus from other closely related homofermentative lactobacilli, especially Lactobacillus gallinarum. This method is based on the amplification by PCR of two peptidoglycan hydrolytic genes, Lhv_0190 and Lhv_0191. These genes are ubiquitous and show high homology at the intra-species level. The PCR method gave two specific PCR products, of 542 bp and 747 bp, for 25 L. helveticus strains coming from various sources. For L. gallinarum, two amplicons were obtained, the specific 542 bp amplicon and another one with a size greater than 1500 bp. No specific PCR products were obtained for 12 other closely related species of lactobacilli, including the Lactobacillus acidophilus complex, Lactobacillus delbrueckii, and Lactobacillus ultunensis. The developed PCR method provided rapid, precise and easy identification of L. helveticus. Moreover, it enabled differentiation between the two closely phylogenetically related species L. helveticus and L. gallinarum. We propose a new method that allows accurate discrimination of Lactobacillus helveticus from other closely related homofermentative lactobacilli, especially Lactobacillus gallinarum. This method is based on the amplification by PCR of two peptidoglycan hydrolytic genes, Lhv_0190 and Lhv_0191. These genes are ubiquitous and show high homology at the intra-species level. The PCR method gave two specific PCR products, of 542 bp and 747 bp, for 25 L. helveticus strains coming from various sources. For L. gallinarum, two amplicons were obtained, the specific 542 bp amplicon and another one with a size greater than 1500 bp. No specific PCR products were obtained for 12 other closely related species of lactobacilli, including the Lactobacillus acidophilus complex, Lactobacillus delbrueckii, and Lactobacillus ultunensis. The developed PCR method provided rapid, precise and easy identification of L. helveticus. Moreover, it enabled differentiation between the two closely phylogenetically related species L. helveticus and L. gallinarum.
dcterms:title
Peptidoglycan Hydrolases as Species-Specific Markers to Differentiate Lactobacillus helveticus from Lactobacillus gallinarum and Other Closely Related Homofermentative Lactobacilli Peptidoglycan Hydrolases as Species-Specific Markers to Differentiate Lactobacillus helveticus from Lactobacillus gallinarum and Other Closely Related Homofermentative Lactobacilli
skos:prefLabel
Peptidoglycan Hydrolases as Species-Specific Markers to Differentiate Lactobacillus helveticus from Lactobacillus gallinarum and Other Closely Related Homofermentative Lactobacilli Peptidoglycan Hydrolases as Species-Specific Markers to Differentiate Lactobacillus helveticus from Lactobacillus gallinarum and Other Closely Related Homofermentative Lactobacilli
skos:notation
RIV/60461373:22330/14:43895925!RIV15-MZE-22330___
n3:aktivita
n10:P
n3:aktivity
P(QI101B090)
n3:cisloPeriodika
4
n3:dodaniDat
n12:2015
n3:domaciTvurceVysledku
n18:6900593
n3:druhVysledku
n5:J
n3:duvernostUdaju
n20:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
36082
n3:idVysledku
RIV/60461373:22330/14:43895925
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Lactobacillus helveticus, Lactobacillus gallinarum, species-specific PCR, peptidoglycan hydrolases
n3:klicoveSlovo
n8:peptidoglycan%20hydrolases n8:species-specific%20PCR n8:Lactobacillus%20helveticus n8:Lactobacillus%20gallinarum
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[543FD5BBCF80]
n3:nazevZdroje
CURRENT MICROBIOLOGY
n3:obor
n6:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
4
n3:projekt
n11:QI101B090
n3:rokUplatneniVysledku
n12:2014
n3:svazekPeriodika
68
n3:tvurceVysledku
Jebavá, Iva Valence, Florence Chuat, Victoria Lortal, Sylvie
n3:wos
000331006200019
s:issn
0343-8651
s:numberOfPages
7
n19:doi
10.1007/s00284-013-0512-5
n15:organizacniJednotka
22330