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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F13%3A43895482%21RIV14-MSM-22330___
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://link.springer.com/article/10.1007%2Fs11240-012-0239-1
dcterms:description
As most cultivars of flax and linseed (Linum usitatissimum L.) are capable of accumulating cadmium (Cd), they are suitable candidates for phytoextraction of the metal from contaminated soils. In an attempt to enhance the phytoextraction capacity of L. usitatissimum through overproduction of an efficient heterologous Cd-binding peptide, we engineered linseed breeding line AGT 917 to constitutively express genetic fusion of alpha-domain of mammalian metallothionein 1a (alpha MT1a) and beta-glucuronidase gus gene under the control of CaMV 35S promoter. An improved transformation protocol was developed, which involved co-cultivation of AGT 917 hypocotyl segments with partially removed epidermis with Agrobacterium suspension for 10 min in the presence of 200 mg l(-1) cellulase. The enzyme treatment increased the transformation efficiency (TE) 1.6-fold as compared to agroinfection without cellulase. Less pronounced impact on TE exerted 100 mg l(-1) acetosyringone, increasing TE 1.3-fold. When tested in soils amended with Cd at 20 and 360 mg kg(-1), the mature alpha MT1a::gus plants accumulated more Cd than parental AGT 917: the stem Cd concentrations in the best performing alpha MT1/2 line were 3.3- and 1.9-fold higher, respectively. Moreover, hypocotyl explants of alpha MT1/2 line showed 1.7-fold higher biomass than those of AGT 917 on media containing 15 mg Cd l(-1), indicating that alpha MT1a::gus did confer higher Cd tolerance to engineered plant. Overproduction of metal-binding peptides thus appears to be a viable strategy for the production of L. usitatissimum with improved phytoremediation capacity. As most cultivars of flax and linseed (Linum usitatissimum L.) are capable of accumulating cadmium (Cd), they are suitable candidates for phytoextraction of the metal from contaminated soils. In an attempt to enhance the phytoextraction capacity of L. usitatissimum through overproduction of an efficient heterologous Cd-binding peptide, we engineered linseed breeding line AGT 917 to constitutively express genetic fusion of alpha-domain of mammalian metallothionein 1a (alpha MT1a) and beta-glucuronidase gus gene under the control of CaMV 35S promoter. An improved transformation protocol was developed, which involved co-cultivation of AGT 917 hypocotyl segments with partially removed epidermis with Agrobacterium suspension for 10 min in the presence of 200 mg l(-1) cellulase. The enzyme treatment increased the transformation efficiency (TE) 1.6-fold as compared to agroinfection without cellulase. Less pronounced impact on TE exerted 100 mg l(-1) acetosyringone, increasing TE 1.3-fold. When tested in soils amended with Cd at 20 and 360 mg kg(-1), the mature alpha MT1a::gus plants accumulated more Cd than parental AGT 917: the stem Cd concentrations in the best performing alpha MT1/2 line were 3.3- and 1.9-fold higher, respectively. Moreover, hypocotyl explants of alpha MT1/2 line showed 1.7-fold higher biomass than those of AGT 917 on media containing 15 mg Cd l(-1), indicating that alpha MT1a::gus did confer higher Cd tolerance to engineered plant. Overproduction of metal-binding peptides thus appears to be a viable strategy for the production of L. usitatissimum with improved phytoremediation capacity.
dcterms:title
Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein
skos:prefLabel
Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein
skos:notation
RIV/60461373:22330/13:43895482!RIV14-MSM-22330___
n5:predkladatel
n18:orjk%3A22330
n6:aktivita
n13:I n13:V
n6:aktivity
I, V
n6:cisloPeriodika
3
n6:dodaniDat
n12:2014
n6:domaciTvurceVysledku
n20:5077184
n6:druhVysledku
n7:J
n6:duvernostUdaju
n16:S
n6:entitaPredkladatele
n11:predkladatel
n6:idSjednocenehoVysledku
72919
n6:idVysledku
RIV/60461373:22330/13:43895482
n6:jazykVysledku
n8:eng
n6:klicovaSlova
Phytoextraction; Metallothionein; Linseed; Flax; Cadmium; Linum usitatissimum
n6:klicoveSlovo
n10:Linum%20usitatissimum n10:Cadmium n10:Phytoextraction n10:Linseed n10:Flax n10:Metallothionein
n6:kodStatuVydavatele
NL - Nizozemsko
n6:kontrolniKodProRIV
[90F1C94AA95F]
n6:nazevZdroje
Plant Cell Tissue and Organ Culture
n6:obor
n17:EF
n6:pocetDomacichTvurcuVysledku
1
n6:pocetTvurcuVysledku
8
n6:rokUplatneniVysledku
n12:2013
n6:svazekPeriodika
112
n6:tvurceVysledku
Větrovcová, Martina Kotrba, Pavel Horáček, Jiří Švábová, Lenka Griga, Miroslav Smýkal, Petr Smýkalová, Iva Vrbová, Miroslava
n6:wos
000315277300006
s:issn
0167-6857
s:numberOfPages
10
n9:doi
10.1007/s11240-012-0239-1
n15:organizacniJednotka
22330