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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F13%3A43893289%21RIV15-MSM-22330___
rdf:type
skos:Concept n13:Vysledek
dcterms:description
The current interest in microalgae as a sustainable source of next generation biofuels and other valuable substances is driving exploration of their use as unique biotechnological production systems. To design and optimise appropriate production strategies, the behaviour of particular microalgal species should be well characterised under different culture conditions. Thus, flow cytometric (FCM) methods, which are already well established in environmental and toxicological studies of microalgae, are also useful for analysing the physiological state of microalgae, and have the potential to contribute to the rapid development of feasible bioprocesses. These methods are commonly based on the examination of intrinsic features of individual cells within a population (such as autofluorescence or size). Cells possessing the desired physiological or morphological features, which are detectable with or without fluorescent staining, are counted or isolated (sorted) using an FCM device. The options for implementation of FCM in the development of biotechnological processes detailed in this review are (i) analysing the chemical composition of biomass, (ii) monitoring cellular enzyme activity and cell viability, and (iii) sorting cells to isolate those overproducing the target compound or for the preparation of axenic cultures. The current interest in microalgae as a sustainable source of next generation biofuels and other valuable substances is driving exploration of their use as unique biotechnological production systems. To design and optimise appropriate production strategies, the behaviour of particular microalgal species should be well characterised under different culture conditions. Thus, flow cytometric (FCM) methods, which are already well established in environmental and toxicological studies of microalgae, are also useful for analysing the physiological state of microalgae, and have the potential to contribute to the rapid development of feasible bioprocesses. These methods are commonly based on the examination of intrinsic features of individual cells within a population (such as autofluorescence or size). Cells possessing the desired physiological or morphological features, which are detectable with or without fluorescent staining, are counted or isolated (sorted) using an FCM device. The options for implementation of FCM in the development of biotechnological processes detailed in this review are (i) analysing the chemical composition of biomass, (ii) monitoring cellular enzyme activity and cell viability, and (iii) sorting cells to isolate those overproducing the target compound or for the preparation of axenic cultures.
dcterms:title
Flow cytometry for the development of biotechnological processes with microalgae Flow cytometry for the development of biotechnological processes with microalgae
skos:prefLabel
Flow cytometry for the development of biotechnological processes with microalgae Flow cytometry for the development of biotechnological processes with microalgae
skos:notation
RIV/60461373:22330/13:43893289!RIV15-MSM-22330___
n4:aktivita
n16:I n16:S
n4:aktivity
I, S
n4:cisloPeriodika
1
n4:dodaniDat
n6:2015
n4:domaciTvurceVysledku
n7:8537003 n7:5648858 n7:4521137
n4:druhVysledku
n14:J
n4:duvernostUdaju
n11:S
n4:entitaPredkladatele
n15:predkladatel
n4:idSjednocenehoVysledku
75313
n4:idVysledku
RIV/60461373:22330/13:43893289
n4:jazykVysledku
n18:eng
n4:klicovaSlova
Single cell physiology; Production proces; Population heterogeneity; Microalga; Fluorescence; Flow cytometry; Cell sorting
n4:klicoveSlovo
n9:Microalga n9:Population%20heterogeneity n9:Production%20proces n9:Single%20cell%20physiology n9:Cell%20sorting n9:Fluorescence n9:Flow%20cytometry
n4:kodStatuVydavatele
CA - Kanada
n4:kontrolniKodProRIV
[292253CC1034]
n4:nazevZdroje
Biotechnology Advances
n4:obor
n17:EI
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
5
n4:rokUplatneniVysledku
n6:2013
n4:svazekPeriodika
31
n4:tvurceVysledku
Kovář, Karin Ličková, Sandra Přibyl, P. Melzoch, Karel Hyka, Petr
s:issn
0734-9750
s:numberOfPages
15
n10:doi
10.1016/j.biotechadv.2012.04.007
n12:organizacniJednotka
22330