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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F12%3A43894815%21RIV13-MZE-22330___
rdf:type
skos:Concept n15:Vysledek
dcterms:description
An increasing incidence of T-2 and HT-2 toxins (T-2 and HT-2) representing group A trichothecenes has been observed in Fusarium infected small grain cereals in the last decade. Quite recently, the existence of glucosides of T-2 and HT-2 has also been proved next to the common parent forms. There is a strong desire to deepen the knowledge of the occurrence ot these conjugates and the possibilities of their analytical determination. A new analytical procedure has been developed for monitoring T-2/HT-2 conjugates in cereal samples. Four different sample preparation methods based on crude acetonitrile:water extraction, QuEChERS, solid phase extraction ind immunoaffinity clean-up, were tested. The latter approach employing dedicated immunoaffinity cross-reactive cartridges was shown to be the best option for selective isolation and pre-concentration of the target conjugated analytes. The samples obtained in this way were examined by ultra-high performance liquid chromatography hyphenated to high resolution tandem mass spectrometry that enabled the confirmation of the presence of conjugated T-2 and HT-2. In addition to mono-glycosylated forms of T-2 and HT-2 detected in naturally contaminated barley, wheat and oats, we have also documented for the first time the existence of diglucosides of HT-2 in barley. An increasing incidence of T-2 and HT-2 toxins (T-2 and HT-2) representing group A trichothecenes has been observed in Fusarium infected small grain cereals in the last decade. Quite recently, the existence of glucosides of T-2 and HT-2 has also been proved next to the common parent forms. There is a strong desire to deepen the knowledge of the occurrence ot these conjugates and the possibilities of their analytical determination. A new analytical procedure has been developed for monitoring T-2/HT-2 conjugates in cereal samples. Four different sample preparation methods based on crude acetonitrile:water extraction, QuEChERS, solid phase extraction ind immunoaffinity clean-up, were tested. The latter approach employing dedicated immunoaffinity cross-reactive cartridges was shown to be the best option for selective isolation and pre-concentration of the target conjugated analytes. The samples obtained in this way were examined by ultra-high performance liquid chromatography hyphenated to high resolution tandem mass spectrometry that enabled the confirmation of the presence of conjugated T-2 and HT-2. In addition to mono-glycosylated forms of T-2 and HT-2 detected in naturally contaminated barley, wheat and oats, we have also documented for the first time the existence of diglucosides of HT-2 in barley.
dcterms:title
Occurrence of mono- and di-glycosylated conjugates of T-2 and HT-2 toxins in naturally contaminated cereals Occurrence of mono- and di-glycosylated conjugates of T-2 and HT-2 toxins in naturally contaminated cereals
skos:prefLabel
Occurrence of mono- and di-glycosylated conjugates of T-2 and HT-2 toxins in naturally contaminated cereals Occurrence of mono- and di-glycosylated conjugates of T-2 and HT-2 toxins in naturally contaminated cereals
skos:notation
RIV/60461373:22330/12:43894815!RIV13-MZE-22330___
n15:predkladatel
n16:orjk%3A22330
n3:aktivita
n20:P
n3:aktivity
P(QH81060)
n3:cisloPeriodika
3
n3:dodaniDat
n9:2013
n3:domaciTvurceVysledku
n7:7726481 n7:2206072 n7:7989253 n7:7597037 n7:4915917 n7:8580588
n3:druhVysledku
n19:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
155601
n3:idVysledku
RIV/60461373:22330/12:43894815
n3:jazykVysledku
n4:eng
n3:klicovaSlova
UPLC-QqTOF MS; high resolution mass spectrometry; T-2 toxin; HT-2 toxin; masked mycotoxins
n3:klicoveSlovo
n10:high%20resolution%20mass%20spectrometry n10:T-2%20toxin n10:HT-2%20toxin n10:UPLC-QqTOF%20MS n10:masked%20mycotoxins
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[C8C28FE01D1F]
n3:nazevZdroje
WORLD MYCOTOXIN JOURNAL
n3:obor
n8:GM
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n14:QH81060
n3:rokUplatneniVysledku
n9:2012
n3:svazekPeriodika
5
n3:tvurceVysledku
Vepříková, Zdenka Džuman, Zbyněk Zachariášová, Milena Hajšlová, Jana Lacina, Ondřej Václavíková, Marta
n3:wos
000308031500004
s:issn
1875-0710
s:numberOfPages
10
n13:doi
10.3920/WMJ2012.1453
n12:organizacniJednotka
22330