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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F12%3A43894787%21RIV13-MSM-22330___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
The development and use of a fast method employing a direct analysis in real time (DART) ion source coupled to high-resolution time-of-flight mass spektrometry (TOFMS) for the quantitative analysis of caffeine in various coffee samples has been demonstrated in this study. A simple sample extraction procedure employing hot water was followed by direct, high-throughput ({1 min per run) examination of the extracts spread on a glass rod under optimized conditions of ambient mass spectrometry, without any prior chromatographic separation. For quantification of caffeine using DART-TOFMS, an external calibration was used. Isotopically labeled caffeine was used to compensate for the variations of the ion intensities of caffeine signal. Recoveries of the DART-TOFMS method were 97% for instant coffee at the spiking levels of 20 and 60 mg/g, respectively, while for roasted ground coffee, the obtained values were 106% and 107% at the spiking levels of 10 and 30 mg/g, respectively. The repeatability of the whole analytical procedure (expressed as relative standard deviation, RSD, %) was {5% for all tested spiking levels and matrices. Since the linearity range of the method was relativem narrow (two orders of magnitude), an optimization of sample dilution prior the DART-TOFMS measurement to avoid saturation of the detector was needed. The development and use of a fast method employing a direct analysis in real time (DART) ion source coupled to high-resolution time-of-flight mass spektrometry (TOFMS) for the quantitative analysis of caffeine in various coffee samples has been demonstrated in this study. A simple sample extraction procedure employing hot water was followed by direct, high-throughput ({1 min per run) examination of the extracts spread on a glass rod under optimized conditions of ambient mass spectrometry, without any prior chromatographic separation. For quantification of caffeine using DART-TOFMS, an external calibration was used. Isotopically labeled caffeine was used to compensate for the variations of the ion intensities of caffeine signal. Recoveries of the DART-TOFMS method were 97% for instant coffee at the spiking levels of 20 and 60 mg/g, respectively, while for roasted ground coffee, the obtained values were 106% and 107% at the spiking levels of 10 and 30 mg/g, respectively. The repeatability of the whole analytical procedure (expressed as relative standard deviation, RSD, %) was {5% for all tested spiking levels and matrices. Since the linearity range of the method was relativem narrow (two orders of magnitude), an optimization of sample dilution prior the DART-TOFMS measurement to avoid saturation of the detector was needed.
dcterms:title
Rapid analysis of caffeine in various coffee samples employing direct analysis in real-time ionization-high-resolution mass spectrometry Rapid analysis of caffeine in various coffee samples employing direct analysis in real-time ionization-high-resolution mass spectrometry
skos:prefLabel
Rapid analysis of caffeine in various coffee samples employing direct analysis in real-time ionization-high-resolution mass spectrometry Rapid analysis of caffeine in various coffee samples employing direct analysis in real-time ionization-high-resolution mass spectrometry
skos:notation
RIV/60461373:22330/12:43894787!RIV13-MSM-22330___
n8:predkladatel
n17:orjk%3A22330
n3:aktivita
n9:Z n9:I
n3:aktivity
I, Z(MSM6046137305)
n3:cisloPeriodika
10
n3:dodaniDat
n16:2013
n3:domaciTvurceVysledku
n12:8580588 n12:8559937 n12:7890451 n12:4423232 n12:3901556 n12:3360792 n12:4604326
n3:druhVysledku
n15:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
164085
n3:idVysledku
RIV/60461373:22330/12:43894787
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Caffeine; Coffee; Direct analysis in real time; Ambient mass spectrometry
n3:klicoveSlovo
n7:Coffee n7:Caffeine n7:Direct%20analysis%20in%20real%20time n7:Ambient%20mass%20spectrometry
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[2A2F556CB26A]
n3:nazevZdroje
Analytical and Bioanalytical Chemistry
n3:obor
n20:GM
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
7
n3:rokUplatneniVysledku
n16:2012
n3:svazekPeriodika
403
n3:tvurceVysledku
Václavík, Lukáš Daňhelová, Hana Přinosilová, Šárka Hradecký, Jaromír Čajka, Tomáš Hajšlová, Jana Riddellová, Kateřina
n3:wos
000305519400012
n3:zamer
n19:MSM6046137305
s:issn
1618-2642
s:numberOfPages
7
n14:doi
10.1007/s00216-012-5820-2
n10:organizacniJednotka
22330